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Product Info Summary
| SKU: | M01847 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-PRDX6/Peroxiredoxin 6 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01847
BM5052 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PRDX6/Peroxiredoxin 6 Rabbit Monoclonal Antibody catalog # M01847. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PRDX6/Peroxiredoxin 6 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01847)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AAGB-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PRDX6
Reactive Species
M01847 is reactive to PRDX6 in Human, Mouse, Rat
Observed Molecular Weight
25 kDa
Calculated molecular weight
25.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01847 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
IP 1:20
Positive Control
WB: human PC-3 whole cell, human K562 whole cell, human Hela whole cell, rat brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue
Validation Images & Assay Conditions
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Western blot analysis of PRDX6 using anti-PRDX6 antibody (M01847).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDX6 antigen affinity purified polyclonal antibody (Catalog # M01847) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRDX6 at approximately 25 kDa. The expected band size for PRDX6 is at 25 kDa.
Specific Publications For Anti-PRDX6/Peroxiredoxin 6 Rabbit Monoclonal Antibody (M01847)
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Customer Q&As
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7 Customer Q&As for Anti-PRDX6/Peroxiredoxin 6 Rabbit Monoclonal Antibody
Question
Is a blocking peptide available for product anti-PRDX6/Peroxiredoxin 6 Rabbit Monoclonal antibody (M01847)?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
We do provide the blocking peptide for product anti-PRDX6/Peroxiredoxin 6 Rabbit Monoclonal antibody (M01847). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-12-16
Question
Our lab want to know about to test anti-PRDX6/Peroxiredoxin 6 Rabbit Monoclonal antibody M01847 on human brain for research purposes, then I may be interested in using anti-PRDX6/Peroxiredoxin 6 Rabbit Monoclonal antibody M01847 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
The products we sell, including anti-PRDX6/Peroxiredoxin 6 Rabbit Monoclonal antibody M01847, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-02
Question
Is this M01847 anti-PRDX6/Peroxiredoxin 6 Rabbit Monoclonal antibody reactive to the isotypes of PRDX6?
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
The immunogen of M01847 anti-PRDX6/Peroxiredoxin 6 Rabbit Monoclonal antibody is A synthesized peptide derived from human PRDX6. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-04
Question
Would anti-PRDX6/Peroxiredoxin 6 Rabbit Monoclonal antibody M01847 work on goat IF with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-09-04
Answer
Our lab technicians have not validated anti-PRDX6/Peroxiredoxin 6 Rabbit Monoclonal antibody M01847 on goat. You can run a BLAST between goat and the immunogen sequence of anti-PRDX6/Peroxiredoxin 6 Rabbit Monoclonal antibody M01847 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat cervix carcinoma erythroleukemia in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-04
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-PRDX6/Peroxiredoxin 6 Rabbit Monoclonal antibody M01847. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-11
Question
Is there a BSA free version of anti-PRDX6/Peroxiredoxin 6 Rabbit Monoclonal antibody M01847 available?
L. Bhatt
Verified customer
Asked: 2018-01-19
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PRDX6/Peroxiredoxin 6 Rabbit Monoclonal antibody M01847 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-01-19
Question
Would anti-PRDX6/Peroxiredoxin 6 Rabbit Monoclonal antibody M01847 work for IF with brain?
P. Brown
Verified customer
Asked: 2015-02-11
Answer
According to the expression profile of brain, PRDX6 is highly expressed in brain. So, it is likely that anti-PRDX6/Peroxiredoxin 6 Rabbit Monoclonal antibody M01847 will work for IF with brain.
Boster Scientific Support
Answered: 2015-02-11
