Product Info Summary
SKU: | M00290 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IP, IHC, WB |
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Product info
Product Name
Anti-Prealbumin TTR Rabbit Monoclonal Antibody
View all Transthyretin/Prealbumin Antibodies
SKU/Catalog Number
M00290
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Prealbumin TTR Rabbit Monoclonal Antibody catalog # M00290. Tested in WB, IHC, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Prealbumin TTR Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00290)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AOFI-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Prealbumin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00290 is reactive to TTR in Human
Applications
M00290 is guaranteed for IP, IHC, WB Boster Guarantee
Observed Molecular Weight
59 kDa
Calculated molecular weight
15.887kDa
Background of Transthyretin/Prealbumin
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Prealbumin gamma expression in human fetal kidney lysate.
Protein Target Info & Infographic
Gene/Protein Information For TTR (Source: Uniprot.org, NCBI)
Gene Name
TTR
Full Name
Transthyretin
Weight
15.887kDa
Superfamily
transthyretin family
Alternative Names
ATTR; HsT2651; PALB; PALBTBPA; Prealbumin; prealbumin, amyloidosis type I; TBPA; thyroxine-binding prealbumin; Transthyretin; TTR TTR ATTR, CTS, CTS1, HEL111, HsT2651, PALB, TBPA, TTN transthyretin transthyretin|epididymis luminal protein 111|prealbumin, amyloidosis type I|thyroxine-binding prealbumin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TTR, check out the TTR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TTR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Prealbumin TTR Rabbit Monoclonal Antibody (M00290)
Hello CJ!
M00290 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Expression of renal cell markers and detection of 3p loss links endolymphatic sac tumor to renal cell carcinoma and warrants careful evaluation to avoid %u2026
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Prealbumin TTR Rabbit Monoclonal Antibody
Question
We have tried in the past anti-Prealbumin Rabbit Monoclonal antibody for WB on retina in the past. I am using human, and We want to use the antibody for IP next. I would like examining retina as well as platelet in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
I looked at the website and datasheets of our anti-Prealbumin Rabbit Monoclonal antibody and it appears that M00290 has been validated on human in both WB and IP. Thus M00290 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-02-25
Question
We are currently using anti-Prealbumin Rabbit Monoclonal antibody M00290 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
The anti-Prealbumin Rabbit Monoclonal antibody (M00290) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-13
Question
Our team were satisfied with the WB result of your anti-Prealbumin Rabbit Monoclonal antibody. However we have been able to see positive staining in retina secreted. cytoplasm. using this antibody. Is that expected? Could you tell me where is TTR supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
From literature, retina does express TTR. Generally TTR expresses in secreted. cytoplasm. Regarding which tissues have TTR expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 1979335
Cerebrospinal fluid, Pubmed ID: 18221012
Corpus callosum, Pubmed ID: 14702039
Liver, Pubmed ID: 1666289, 2015890, 3022108, 6093805, 15489334, 24275569
Plasma, Pubmed ID: 14760718, 16335952
Platelet, Pubmed ID: 12665801
Retina, Pubmed ID: 10328977
Boster Scientific Support
Answered: 2020-01-13
Question
What is the epitope for M00290?
Verified customer
Asked: 2019-11-27
Answer
The immunogen sequence for the Anti-Prealbumin TTR Rabbit Monoclonal Antibody (M00290) is GPTGTGESKC.
Boster Scientific Support
Answered: 2019-12-03
Question
We have seen staining in human cajal-retzius cell fetal brain cortex. Any tips? Is anti-Prealbumin Rabbit Monoclonal antibody supposed to stain cajal-retzius cell fetal brain cortex positively?
S. Singh
Verified customer
Asked: 2017-06-01
Answer
Based on literature cajal-retzius cell fetal brain cortex does express TTR. Based on Uniprot.org, TTR is expressed in pigmented layer of retina, liver, retina, corpus callosum, platelet, brain, cajal-retzius cell fetal brain cortex, plasma, cerebrospinal fluid, among other tissues. Regarding which tissues have TTR expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 1979335
Cerebrospinal fluid, Pubmed ID: 18221012
Corpus callosum, Pubmed ID: 14702039
Liver, Pubmed ID: 1666289, 2015890, 3022108, 6093805, 15489334, 24275569
Plasma, Pubmed ID: 14760718, 16335952
Platelet, Pubmed ID: 12665801
Retina, Pubmed ID: 10328977
Boster Scientific Support
Answered: 2017-06-01