Anti-PRKD2 Antibody Picoband®

Western blot analysis of PRKD2 using anti-PRKD2 antibody (A04056-2).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human SIHA whole cell lysates,
Lane 4: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKD2 antigen affinity purified polyclonal antibody (A04056-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PRKD2 at approximately 105 kDa. The expected band size for PRKD2 is at 97 kDa.

IF analysis of PRKD2 using anti-PRKD2 antibody (A04056-2).
PRKD2 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PRKD2 Antibody (A04056-2) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Immunoprecipitating (IP) PRKD2 in HEL whole cell lysate.
Western blot analysis of PRKD2 using anti-PRKD2 antibody (A04056-2);
Lane 1: HEL whole cell lysates (30ug);
Lane 2: Rabbit control IgG instead of anti-PRKD2 antibody in HEL whole cell lysate;
Lane 3: anti-PRKD2 antibody (2μg) + HEL whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PRKD2 antigen affinity purified polyclonal antibody (A04056-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for PRKD2 at approximately 105 kDa. The expected band size for PRKD2 is at 97 kDa.