Product Info Summary
SKU: | M01486-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Monkey, Rat |
Host: | Mouse |
Application: | ELISA, Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-PRMT4 CARM1 Monoclonal Antibody
SKU/Catalog Number
M01486-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-PRMT4 CARM1 Monoclonal Antibody catalog # M01486-1. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Monkey, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PRMT4 CARM1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01486-1)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
3H2
Isotype
IgG
Immunogen
Purified recombinant fragment of human PRMT4 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M01486-1 is reactive to CARM1 in Human, Monkey, Rat
Applications
M01486-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
65.854kDa
Background of CARM1
TRPC6, also known as TRP6, short transient receptor potential channel 6 and transient receptor potential cation channel subfamily C member 6, is thought to form a receptor-activated non-selective calcium permeant cation channel. TRPC6 is probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. It is activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C and may not to be activated by intracellular calcium store depletion. Defects in this gene are a cause of focal segmental glomerulosclerosis (FSGS). Expression of this protein has been reported in tissues such as placenta, lung, spleen, ovary, small intestine, and renal podocytes. Immunohistochemistry studies using polyclonal antibodies to this target have shown moderate to strong staining in cell types such as neurons, breast, respiratory, squamous and prostate epithelium, epidermis, placental trophoblasts, dendritic cells, and subsets of immune cells, and faint to moderate staining of adrenal, colon, ganglion cells, hepatocytes, heart, and testis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Validation Images & Assay Conditions
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Flow cytometric analysis of Lovo cells using PRMT4 monoclonal antibody (green) and negative control (purple).
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Immunofluorescence analysis of Hela cells using PRMT4 monoclonal antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Immunohistochemistry analysis of paraffin-embedded breast cancer tissues (left) and ovarian cancer tissues (right) with DAB staining using PRMT4 monoclonal antibody.
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Western blot analysis using PRMT4 monoclonal antibody against MCF-7 (1) , HeLa (2) , NIH/3T3 (3) , HL-60 (4) , LNcap (5) , Jurkat (6) , PC-3 (7) , Cos7 (8) , and PC-12 (9) cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For CARM1 (Source: Uniprot.org, NCBI)
Gene Name
CARM1
Full Name
Histone-arginine methyltransferase CARM1
Weight
65.854kDa
Superfamily
class I-like SAM-binding methyltransferase superfamily
Alternative Names
Carm1; coactivator-associated arginine methyltransferase 1EC 2.1.1.-; EC 2.1.1; histone-arginine methyltransferase CARM1; PRMT4; PRMT4EC 2.1.1.125; Protein arginine N-methyltransferase 4 CARM1 PRMT4 coactivator associated arginine methyltransferase 1 histone-arginine methyltransferase CARM1|protein arginine N-methyltransferase 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CARM1, check out the CARM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CARM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PRMT4 CARM1 Monoclonal Antibody (M01486-1)
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No publications found for M01486-1
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