Anti-PRMT5 Antibody

PRMT5 antibody

Boster Bio Anti-PRMT5 Antibody catalog # A00635-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A00635-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, WB

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Product Name

Anti-PRMT5 Antibody

View all PRMT5 Antibodies

SKU/Catalog Number

A00635-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PRMT5 Antibody catalog # A00635-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PRMT5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00635-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PRMT5 recombinant protein (Position: R18-A552).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00635-1 is reactive to PRMT5 in Human, Mouse, Rat

Applications

A00635-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

72.684kDa

Background of PRMT5

Protein arginine N-methyltransferase 5 is an enzyme that in humans is encoded by the PRMT5 gene. It is mapped to 14q11.2. This gene encodes an enzyme that belongs to the methyltransferase family. The encoded protein catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. This gene plays a role in several cellular processes, including transcriptional regulation, and the assembly of small nuclear ribonucleoproteins. A pseudogene of this gene has been defined on chromosome 4. Alternative splicing results in multiple transcript variants encoding different isoforms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.5-1μg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For PRMT5 (Source: Uniprot.org, NCBI)

Gene Name

PRMT5

Full Name

Protein arginine N-methyltransferase 5

Weight

72.684kDa

Superfamily

class I-like SAM-binding methyltransferase superfamily

Alternative Names

EC 2.1.1.-; EC 2.1.1.125; Histone-arginine N-methyltransferase PRMT5; HMT1 hnRNP methyltransferase-like 5,72 kDa ICln-binding protein; HRMT1L5; IBP72; Jak-binding protein 1; JBP1; protein arginine methyltransferase 5; protein arginine N-methyltransferase 5; Shk1 kinase-binding protein 1 homolog; skb1 (S. pombe) homolog; SKB1 homolog (S. pombe); SKB1; SKB1HsSKB1 homolog PRMT5 HRMT1L5, HSL7, IBP72, JBP1, SKB1, SKB1Hs protein arginine methyltransferase 5 protein arginine N-methyltransferase 5|72 kDa ICln-binding protein|HMT1 hnRNP methyltransferase-like 5|SKB1 homolog|histone-arginine N-methyltransferase PRMT5|jak-binding protein 1|shk1 kinase-binding protein 1 homolog

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PRMT5, check out the PRMT5 Infographic

PRMT5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PRMT5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-PRMT5 Antibody

Question

We are currently using anti-PRMT5 antibody A00635-1 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?

E. Baker

Verified customer

Asked: 2014-12-02

Answer

The anti-PRMT5 antibody (A00635-1) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-12-02

Order DetailsPrice
A00635-1

100μg

$370
A00635-1-10ug

10μg sample (liquid)

$99
A00635-1-Biotin

100 μg Biotin conjugated

$570
A00635-1-Cy3

100 μg Cy3 conjugated

$570
A00635-1-Dylight488

100 μg Dylight488 conjugated

$570
A00635-1-Dylight550

100 μg Dylight550 conjugated

$570
A00635-1-Dylight594

100 μg Dylight594 conjugated

$570
A00635-1-FITC

100 μg FITC conjugated

$570
A00635-1-HRP

100 μg HRP conjugated

$570
A00635-1-APC

100 μg APC conjugated

$670
A00635-1-PE

100 μg PE conjugated

$670
A00635-1-carrier-free

Carrier Free

$370

More conjugates/formats

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A00635-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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