Anti-PRMT5 Rabbit Monoclonal Antibody

PRMT5 antibody

Boster Bio Anti-PRMT5 Rabbit Monoclonal Antibody catalog # M00635. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M00635
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-PRMT5 Rabbit Monoclonal Antibody

View all PRMT5 Antibodies

SKU/Catalog Number

M00635

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-PRMT5 Rabbit Monoclonal Antibody catalog # M00635. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PRMT5 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00635)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

DCE-16

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human PRMT5

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00635 is reactive to PRMT5 in Human, Mouse, Rat

Applications

M00635 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

85 kDa

Calculated molecular weight

72.684kDa

Background of PRMT5

Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:5000-1:10000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For PRMT5 (Source: Uniprot.org, NCBI)

Gene Name

PRMT5

Full Name

Protein arginine N-methyltransferase 5

Weight

72.684kDa

Superfamily

class I-like SAM-binding methyltransferase superfamily

Alternative Names

EC 2.1.1.-; EC 2.1.1.125; Histone-arginine N-methyltransferase PRMT5; HMT1 hnRNP methyltransferase-like 5,72 kDa ICln-binding protein; HRMT1L5; IBP72; Jak-binding protein 1; JBP1; protein arginine methyltransferase 5; protein arginine N-methyltransferase 5; Shk1 kinase-binding protein 1 homolog; skb1 (S. pombe) homolog; SKB1 homolog (S. pombe); SKB1; SKB1HsSKB1 homolog PRMT5 HRMT1L5, HSL7, IBP72, JBP1, SKB1, SKB1Hs protein arginine methyltransferase 5 protein arginine N-methyltransferase 5|72 kDa ICln-binding protein|HMT1 hnRNP methyltransferase-like 5|SKB1 homolog|histone-arginine N-methyltransferase PRMT5|jak-binding protein 1|shk1 kinase-binding protein 1 homolog

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PRMT5, check out the PRMT5 Infographic

PRMT5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PRMT5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-PRMT5 Rabbit Monoclonal Antibody

Question

I see that the anti-PRMT5 Rabbit Monoclonal antibody M00635 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-08-07

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-08-07

Question

Will anti-PRMT5 Rabbit Monoclonal antibody M00635 work for WB with testis?

Verified Customer

Verified customer

Asked: 2019-07-17

Answer

According to the expression profile of testis, PRMT5 is highly expressed in testis. So, it is likely that anti-PRMT5 Rabbit Monoclonal antibody M00635 will work for WB with testis.

Boster Scientific Support

Answered: 2019-07-17

Question

Is a blocking peptide available for product anti-PRMT5 Rabbit Monoclonal antibody (M00635)?

Verified Customer

Verified customer

Asked: 2019-01-18

Answer

We do provide the blocking peptide for product anti-PRMT5 Rabbit Monoclonal antibody (M00635). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-01-18

Question

Is this M00635 anti-PRMT5 Rabbit Monoclonal antibody reactive to the isotypes of PRMT5?

J. Li

Verified customer

Asked: 2019-01-18

Answer

The immunogen of M00635 anti-PRMT5 Rabbit Monoclonal antibody is A synthesized peptide derived from human PRMT5. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-01-18

Question

I was wanting to use your anti-PRMT5 Rabbit Monoclonal antibody for WB for mouse testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse testis identification?

Verified Customer

Verified customer

Asked: 2018-08-06

Answer

You can see on the product datasheet, M00635 anti-PRMT5 Rabbit Monoclonal antibody has been tested for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse testis in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-08-06

Question

Would M00635 anti-PRMT5 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

M. Edwards

Verified customer

Asked: 2013-08-01

Answer

You can see on the product datasheet, M00635 anti-PRMT5 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2013-08-01

Order DetailsPrice
M00635

100μL

$370
M00635-30ul

30μL sample

$99
M00635-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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M00635
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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