Click image to see more details
-
-
-
-
-
+2
Product Info Summary
| SKU: | M00635 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-PRMT5 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00635
BM4294 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PRMT5 Rabbit Monoclonal Antibody catalog # M00635. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PRMT5 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00635)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DCE-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PRMT5
Reactive Species
M00635 is reactive to PRMT5 in Human, Mouse, Rat
Observed Molecular Weight
71 kDa
Calculated molecular weight
72.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00635 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-5000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:20
Positive Control
WB: human Hela whole cell, human 293T whole cell, human MCF-7 whole cell, human K562 whole cell, rat spleen tissue, rat brain tissue, mouse spleen tissue, mouse brain tissue
IHC: human testis tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of PRMT5 using anti-PRMT5 antibody (M00635).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat spleen tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse spleen tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRMT5 antigen affinity purified monoclonal antibody (Catalog # M00635) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRMT5 at approximately 71 kDa. The expected band size for PRMT5 is at 71,73 kDa.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human testis, using PRMT5 Antibody .
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:150 dilution.
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:150 dilution.
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Specific Publications For Anti-PRMT5 Rabbit Monoclonal Antibody (M00635)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-PRMT5 Rabbit Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-PRMT5 Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Anti-PRMT5 Rabbit Monoclonal Antibody
Question
I see that the anti-PRMT5 Rabbit Monoclonal antibody M00635 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-08-07
Question
Will anti-PRMT5 Rabbit Monoclonal antibody M00635 work for WB with testis?
Verified Customer
Verified customer
Asked: 2019-07-17
Answer
According to the expression profile of testis, PRMT5 is highly expressed in testis. So, it is likely that anti-PRMT5 Rabbit Monoclonal antibody M00635 will work for WB with testis.
Boster Scientific Support
Answered: 2019-07-17
Question
Is a blocking peptide available for product anti-PRMT5 Rabbit Monoclonal antibody (M00635)?
Verified Customer
Verified customer
Asked: 2019-01-18
Answer
We do provide the blocking peptide for product anti-PRMT5 Rabbit Monoclonal antibody (M00635). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-01-18
Question
Is this M00635 anti-PRMT5 Rabbit Monoclonal antibody reactive to the isotypes of PRMT5?
J. Li
Verified customer
Asked: 2019-01-18
Answer
The immunogen of M00635 anti-PRMT5 Rabbit Monoclonal antibody is A synthesized peptide derived from human PRMT5. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-01-18
Question
I was wanting to use your anti-PRMT5 Rabbit Monoclonal antibody for WB for mouse testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse testis identification?
Verified Customer
Verified customer
Asked: 2018-08-06
Answer
You can see on the product datasheet, M00635 anti-PRMT5 Rabbit Monoclonal antibody has been tested for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-06
Question
Would M00635 anti-PRMT5 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Edwards
Verified customer
Asked: 2013-08-01
Answer
You can see on the product datasheet, M00635 anti-PRMT5 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-08-01
