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- Table of Contents
CASP3 antibody (M00334-1) performs well in Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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Immunofluorescent analysis of Hela cells, using pro Caspase 3 Antibody.
Designing a Western blot for CASP3? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the CASP3 Western Blot GuideBundle Save
Anti-pro Caspase 3 CASP3 Rabbit Monoclonal Antibody
CASP3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse | Rabbit | Flow Cytometry, IP, IF, IHC, ICC, WB |
Tested Applications
CASP3 antibody was positively detected by WB in:
Jurkat cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CASP3 antibody was positively detected by IHC in:
human colon tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
CASP3 antibody was positively detected by ICC/IF in:
Hela cell
Recommend Dilution
Application
Dilution
Species
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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IHC
IF
IP
FC
Human
Tested
Recommend
Recommend
Recommend
Mouse
Recommend
Recommend
Product Information
Product Name
Anti-pro Caspase 3 CASP3 Rabbit Monoclonal Antibody
View all CASP3 Antibodies
View all CASP3 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
GAG-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human pro Caspase 3
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
35 kDa
Calculated Molecular Weight
31.6 kDa
Cite This Product
Anti-pro Caspase 3 CASP3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00334-1)
Protocols
Western Blot protocol for CASP3 antibody
Immunohistochemistry (IHC) protocol for CASP3 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for CASP3 antibody
Publications
Application:
Species:
Downregulation of long non-coding RNA Opa interacting protein 5-antisense RNA 1 inhibits breast cancer progression by targeting sex-determining region Y-box 2 by microRNA-129-5p upregulation
Published: 2018-11-15
Journal: CANCER SCIENCE
Application: WB
Impact Factor: 4.37
Species: Human
PMID: 30443959
microRNA-129-5p suppresses Adriamycin resistance in breast cancer by targeting SOX2
Published: 2018-05-23
Journal: ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
Application: WB
Impact Factor: 3.12
Species: Human
PMID: 29802821
Review
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Product Promise
We promise Anti-pro Caspase 3 CASP3 Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00334-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 19, 2026