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CASP3 antibody (M00334) performs well in Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 26 Publications

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Immunofluorescent analysis of A673 cells, using pro Caspase 3 Antibody.

Designing a Western blot for CASP3? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the CASP3 Western Blot Guide

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Anti-pro Caspase 3 CASP3 Rabbit Monoclonal Antibody

Name: Anti-pro Caspase 3 CASP3 Rabbit Monoclonal Antibody
Brand: Boster Bio
SKU: M00334
Price: 370 USD
Availability: InStock
Rating: 100 (1 reviews)

CASP3 antibody

26 Publications|

1 Review

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Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	Flow Cytometry, IP, IF, IHC, ICC, WB

Tested Applications

CASP3 antibody was positively detected by WB in:

human Hela whole cell, human Jurkat whole cell, human HepG2 whole cell, human 293T whole cell, rat C6 whole cell, rat RH35 whole cell, mouse NIH/3T3 whole cell, mouse Neuro-2a whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

CASP3 antibody was positively detected by ICC/IF in:

A673 cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Recommend

Rat

Tested

Recommend

Mouse

Tested

Recommend

Product Information

Product Name

Anti-pro Caspase 3 CASP3 Rabbit Monoclonal Antibody
View all CASP3 Antibodies

Form

Liquid

Uniprot

P42574

Gene ID

836

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

AOB-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human pro Caspase 3

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

30 kDa

Calculated Molecular Weight

31.6 kDa

Cite This Product

Anti-pro Caspase 3 CASP3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00334)

Protocols

Western Blot protocol for CASP3 antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for CASP3 antibody

Review Protocol

Publications

Redox-sensitive hyaluronic acid-based glycopeptide nanoparticles: Dual-action platform for mitochondrial disruption and tumor suppression

Published: 2025-10-26

Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Application: WB

Impact Factor: 8.50

Species: Mouse

PMID: 41151716

SKU	M00334
Application	Western Blot
Sample	PC-12 (rat adrenal pheochromocytoma cells, highly differentiated)
Sample Processing Description	① Normal PC12 cells (rat adrenal pheochromocytoma cells, highly differentiated)② PC12 cells are treated with β-amyloid (Aβ) to establish an in vitro Alzheimer's disease (AD) model.③ The AD in vitro model is treated with the therapeutic drug.
Other Reagents	RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody	pro Caspase 3 CASP3 Rabbit Monoclonal Antibody
Primary Incubation	1:3000, overnight at 4 ℃
Secondary Antibody	HRP-conjugated goat anti-rabbit IgG
Secondary Incubation	1:10000, 1 hour in RT
Detection	Substrate: ECL substrate; Image system: ChemiDoc MP
Results Summary	① Normal PC-12 cells (rat adrenal pheochromocytoma cells (highly differentiated)) ② PC12 cells were induced with β-amyloid protein (Aβ) to establish an in vitro model of Alzheimer's disease (AD) ③ The AD in vitro model was treated with therapeutic drugs. Experimental results showed clear target bands, and the total expression level of P65 protein was upregulated after modeling, and downregulated after drug treatment.

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Free Secondary Antibody

Anti-pro Caspase 3 CASP3 Rabbit Monoclonal Antibody

CASP3 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Redox-sensitive hyaluronic acid-based glycopeptide nanoparticles: Dual-action platform for mitochondrial disruption and tumor suppression

Structural characteristics and anti-tumor effect of low molecular weight Dendrobium officinale polysaccharides by reconstructing tumor microenvironment

Development of recombinant protein-based nanoparticle systems for inducing tumor cell apoptosis: In vitro evaluation of their cytotoxic and apoptotic effects on cancer cells

Water Extract of Rice False Smut Balls Activates Nrf2/HO-1 and Apoptosis Pathways, Causing Liver Injury

“Cocktail” anti-tumor strategy through enhanced antigen exposure with photothermal/chemodynamic therapy

Qingxin kaiqiao fang ameliorates memory impairment and inhibits apoptosis in APP/PS1 double transgenic mice through the MAPK pathway

Applications of daunorubicin-loaded PLGA-PLL-PEG-Tf nanoparticles in hematologic malignancies: an in vitro and in vivo evaluation

miR-466 Contributes to the Enhanced Antitumor Effect of Bortezomib on Non-Small-Cell Lung Cancer by Inhibiting CCND1

The Delivery Materials with Chemotherapy Drugs for Treatment of the Positive Margin in Solid Tumors

Novel lncRNA XLOC_032768 protects against renal tubular epithelial cells apoptosis in renal ischemia–reperfusion injury by regulating FNDC3B/TGF-β1

Cyanidin-3-glucoside protects liver from oxidative damage through AMPK/Nrf2 mediated signaling pathway in vivo and in vitro

COX-2-PGE2 signaling pathway contributes to hippocampal neuronal injury and cognitive impairment in PTZ-kindled epilepsy mice

Effects of rosmarinic acid on immunoregulatory activity and hepatocellular carcinoma cell apoptosis in H22 tumor-bearing mice

The study of effect and mechanism of 630-nm laser on human lung adenocarcinoma cell xenograft model in nude mice mediated by hematoporphyrin derivatives

Protective effects of pentadecapeptide derived from Cyclaina sinensis against cyclophosphamide-induced hepatotoxicity

Extraction, Characterization, Antitumor and Immunological Activities of Hemicellulose Polysaccharide from Astragalus radix Herb Residue

Supplementation of Saponins from Leaves of Panax quinquefolius Mitigates Cisplatin-Evoked Cardiotoxicity via Inhibiting Oxidative Stress-Associated Inflammation and Apoptosis in Mice

Essential oil from Fructus Alpinia zerumbet (fruit of Alpinia zerumbet (Pers.) Burtt.et Smith) protected against aortic endothelial cell injury and inflammation in vitro and in vivo

Total flavonoids, extracted from Polygonum knotweed L, exert beneficial hepatoprotection against liver injury

MicroRNA-200a suppresses prostate cancer progression through BRD4/AR signaling pathway

Synergistic anticancer and antibacterial activities of cordycepin and selected natural bioactive compounds

Exosomes Derived from miR-214-Enriched Bone Marrow-Derived Mesenchymal Stem Cells Regulate Oxidative Damage in Cardiac Stem Cells by Targeting CaMKII

Resveratrol induces apoptosis in SGC‑7901 gastric cancer cells

Mycophenolate Mofetil Protects Septic Mice via the Dual Inhibition of Inflammatory Cytokines and PD-1

The antitumor effect of folic acid conjugated-Auricularia auricular polysaccharide-cisplatin complex on cervical carcinoma cells in nude mice

In vitro and in vivo antitumor effects of acetylshikonin isolated from Arnebia euchroma (Royle) Johnst (Ruanzicao) cell suspension cultures

Review

Have you used Anti-pro Caspase 3 CASP3 Rabbit Monoclonal Antibody?

1 Review For Anti-pro Caspase 3 CASP3 Rabbit Monoclonal Antibody

Using Anti‑NF‑κB p65 antibody (SKU: M00334), WB of normal, Aβ‑induced AD‑model and drug‑treated PC‑12 cells shows clear target bands. Total p65 was up‑regulated upon Aβ stimulation and decreased after drug treatment.

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