Anti-Progesterone Receptor/PGR Antibody Picoband™

Product Name

Anti-Progesterone Receptor/PGR Antibody Picoband™

View all PGR Antibodies

SKU/Catalog Number

PB9009

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Progesterone Receptor/PGR Antibody Picoband™ catalog # PB9009. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Progesterone Receptor/PGR Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9009)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Progesterone Receptor recombinant protein (Position: M595-K933). Human Progesterone Receptor shares 95% and 94% amino acid (aa) sequences identity with mouse and rat Progesterone Receptor, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9009 is reactive to PGR in Human

Applications

PB9009 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

90 kDa (PR-A), 118 kDa (PR-B)

Calculated molecular weight

98.981kDa

Background of PGR

The progesterone receptor (PR) is an intracellular steroid receptor that specially binds progesterone in humans. PR has been a member of the steroid receptor superfamily. It is encoded by a single PGR gene residing on chromosome 11q22. The PGR gene uses separate promoters and translational start sites to produce 2 isoforms, PRA and PRB, which are identical except for an additional 165 amino acids present only in the N terminus of PRB. It can be observed in human breast tissues. The proteins function as dimeric molecules in nuclei to regulate the transcription of target genes in a ligand-responsive manner.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. IHC analysis of Progesterone Receptor using anti-Progesterone Receptor antibody (PB9009).
Progesterone Receptor was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Progesterone Receptor Antibody (PB9009) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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Figure 2. Western blot analysis of Progesterone Receptor using anti-Progesterone Receptor antibody (PB9009).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human T-47D whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Progesterone Receptor antigen affinity purified polyclonal antibody (Catalog # PB9009) at 0.1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Progesterone Receptor at approximately 90 KD (PR-A), 118KD (PR-B). The expected band size for Progesterone Receptor is at 99KD.

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Figure 3. IF analysis of Progesterone Receptor using anti-Progesterone Receptor antibody (PB9009).
Progesterone Receptor was detected in immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-Progesterone Receptor Antibody (PB9009) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For PGR (Source: Uniprot.org, NCBI)

Alternative Names

NR3C3; NR3C3PRNuclear receptor subfamily 3 group C member 3; PGR; Progesterone R; progesterone receptor; ProgesteroneR PGR NR3C3, PR progesterone receptor progesterone receptor|nuclear receptor subfamily 3 group C member 3

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In this infographic, you will see the following information for PGR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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