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Product Info Summary
| SKU: | A10603 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Proline-rich protein 5-like PRR5L Antibody
SKU/Catalog Number
A10603
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Proline-rich protein 5-like PRR5L Antibody (Catalog # A10603). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
PRR5L antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Proline-rich protein 5-like PRR5L Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10603)
Host
Rabbit
Contents
PRR5L Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
PRR5L antibody was raised against a 17 amino acid synthetic peptide near the center of human PRR5L. The immunogen is located within amino acids 220 - 270 of PRR5L.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least three isoforms of PRR5L are known to exist; this antibody will recognize only isoforms a and b. PRR5L antibody will not cross-react with PPR5.
Reactive Species
A10603 is reactive to Prr5l in Human, Mouse
Applications
A10603 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
40.982kDa
Background of Prr5l
Proline-rich protein 5-like (PRR5L), also known as Protor-2, is structurally similar to PRR5 and also binds to the mTOR complex 2 (mTORC2). PRR5L is not required for mTORC2 integrity or kinase activity, but dissociates from mTORC2 upon knockdown of tuberous sclerosis complex 1 (TSC1) and TSC2. Knockdown of PRR5L reduced apoptosis in mTORC2 deficient cells as well as cells where mTOR was active, suggesting that mTORC2-dissociated PRR5L may promote apoptosis, and thus play a role in the balance between cell growth and cell death.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PRR5L antibody can be used for detection of PRR5L by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of PRR5L in 3T3 cell lysate with PRR5L antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide
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Immunocytochemistry of PPR5L in 293 cells with PPR5L antibody at 10 μg/mL.
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Immunofluorescence of PPR5L in3T3 cells with PPR5L antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For Prr5l (Source: Uniprot.org, NCBI)
Gene Name
Prr5l
Full Name
Proline-rich protein 5-like
Weight
40.982kDa
Superfamily
PROTOR family
Alternative Names
FLJ14213MGC16218; FLJ22630; proline rich 5 like; Protein observed with Rictor-2; protor-2; PROTOR2proline-rich protein 5-like
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Prr5l, check out the Prr5l Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Prr5l: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Proline-rich protein 5-like PRR5L Antibody (A10603)
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No publications found for A10603
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