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Product Info Summary
| SKU: | M01767-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-PROM1/CD133 Antibody Picoband® (monoclonal, 8B6)
SKU/Catalog Number
M01767-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PROM1/CD133 Antibody Picoband® (monoclonal, 8B6) catalog # M01767-3. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PROM1/CD133 Antibody Picoband® (monoclonal, 8B6) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01767-3)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Monoclonal
Clone Number
8B6
Isotype
Mouse IgG2a
Immunogen
E.coli-derived human PROM1/CD133 recombinant protein (Position: P531-H865). HumanPROM1/CD133 shares 61% amino acid (aa) sequence identity with mouse PROM1/CD133.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M01767-3 is reactive to PROM1 in Human, Mouse
Observed Molecular Weight
120 kDa
Calculated molecular weight
97.2 kDa
Background of PROM1
Prominin-1, also known as CD133, is a glycoprotein that in humans is encoded by the PROM1 gene. It is mapped to 4p15.32. Prominin-1 is a member of pentaspan transmembrane glycoproteins (5-transmembrane, 5-TM), which specifically localize to cellular protrusions. This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. It has been proposed to act as an organizer of cell membrane topology. Prominin-1 was expressed not only on metastatic colon cancer cells, but also on differentiated colonic epithelium in both adult mice and humans.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01767-3 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human CACO-2 whole cell, human SW620 whole cell, mouse kidney tissue
FCM: CACO-2 cell
Validation Images & Assay Conditions
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Western blot analysis of PROM1/CD133 using anti-PROM1/CD133 antibody (M01767-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human CACO-2 whole cell lysates,
Lane 2: human SW620 whole cell lysates,
Lane 3: mouse kidney tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PROM1/CD133 antigen affinity purified monoclonal antibody (Catalog # M01767-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PROM1/CD133 at approximately 120KD. The expected band size for PROM1/CD133 is at 120KD.
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Flow Cytometry analysis of CACO-2 cells using anti-PROM1/CD133 antibody (M01767-3).
Overlay histogram showing CACO-2 cells stained with M01767-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with mouse anti- PROM1/CD133 Antibody (M01767-3, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-PROM1/CD133 Antibody Picoband® (monoclonal, 8B6) (M01767-3)
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