Product Info Summary
SKU: | A03637 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Prostate Apoptosis Response Protein-4 PAWR Antibody
SKU/Catalog Number
A03637
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Prostate Apoptosis Response Protein-4 PAWR Antibody (Catalog # A03637). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Prostate Apoptosis Response Protein-4 PAWR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03637)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human prostate apoptosis response protein-4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03637 is reactive to PAWR in Human, Mouse, Rat
Applications
A03637 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
36.568kDa
Background of Pawr
Pro-apoptopic protein capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. Induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappa-B transcriptional activity. Inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. Down-regulates the anti-apoptotic protein BCL2 via its interaction with WT1. Seems also to be a transcriptional repressor by itself. May be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1.
Hongzhe Li, J. Biol. Chem., Feb 2007; 282: 4943 - 4950.
Wuhan Xiao, Cancer Res., Aug 2003; 63: 4698 - 4704.
Thierry Virolle, J. Biol. Chem., Mar 2003; 278: 11802 - 11810.
Derek J. Richard, Genes & Dev., Feb 2001; 15: 328.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from 293 cells(Lane 2), using Prostate Apoptosis Response Protein-4 antiobdy A03637. The lane on the left is treated with synthesized peptide.
Click image to see more details
Western blot analysis of extracts from NIH/3T3 cells, using Prostate Apoptosis Response Protein-4 antibody A03637.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Prostate Apoptosis Response Protein-4 antibody A03637.
Protein Target Info & Infographic
Gene/Protein Information For PAWR (Source: Uniprot.org, NCBI)
Gene Name
PAWR
Full Name
PRKC apoptosis WT1 regulator protein
Weight
36.568kDa
Alternative Names
Par-4; PAR4prostate apoptosis response protein 4; PRKC apoptosis WT1 regulator protein; PRKC, apoptosis, WT1, regulator; Prostate apoptosis response 4 protein; prostate apoptosis response protein PAR-4; transcriptional repressor PAR4; WT1-interacting protein PAWR PAR4, Par-4 pro-apoptotic WT1 regulator PRKC apoptosis WT1 regulator protein|PRKC, apoptosis, WT1, regulator|WT1-interacting protein|prostate apoptosis response protein 4|prostate apoptosis response protein PAR-4|prostate apoptosis response-4|transcriptional repressor PAR4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PAWR, check out the PAWR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PAWR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Prostate Apoptosis Response Protein-4 PAWR Antibody (A03637)
Hello CJ!
No publications found for A03637
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Prostate Apoptosis Response Protein-4 PAWR Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Prostate Apoptosis Response Protein-4 PAWR Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-Prostate Apoptosis Response Protein-4 PAWR Antibody
Question
We are currently using anti-Prostate Apoptosis Response Protein-4 antibody A03637 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-03-15
Answer
The anti-Prostate Apoptosis Response Protein-4 antibody (A03637) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-03-15
Question
Will A03637 anti-Prostate Apoptosis Response Protein-4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-01-22
Answer
As indicated on the product datasheet, A03637 anti-Prostate Apoptosis Response Protein-4 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-01-22
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-Prostate Apoptosis Response Protein-4 antibody A03637. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-07-05
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-05
Question
Is a blocking peptide available for product anti-Prostate Apoptosis Response Protein-4 antibody (A03637)?
B. Williams
Verified customer
Asked: 2016-11-15
Answer
We do provide the blocking peptide for product anti-Prostate Apoptosis Response Protein-4 antibody (A03637). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-11-15
Question
Can you help my question with product A03637, anti-Prostate Apoptosis Response Protein-4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
O. Wu
Verified customer
Asked: 2013-07-02
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03637 anti-Prostate Apoptosis Response Protein-4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-07-02