Anti-Protein AATF AATF Antibody

Product Name

Anti-Protein AATF AATF Antibody

View all AATF Antibodies

SKU/Catalog Number

A03945

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Protein AATF AATF Antibody (Catalog # A03945). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

AATF antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Protein AATF AATF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03945)

Host

Rabbit

Contents

AATF Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

AATF antibody was raised against a 12 amino acid synthetic peptide from near the carboxy terminus of human AATF. The immunogen is located within the last 50 amino acids of AATF.

Reactive Species

A03945 is reactive to AATF in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

63.1 kDa

Background of AATF

AATF (apoptosis antagonizing transcription factor) was initially discovered as an interaction partner of ZIP kinase (ZIPK), a member of death-associated protein (DAP) kinase family of pro-apoptotic serine/threonine kinases. AATF is a phosphoprotein containing an acidic region and a putative leucine zipper domain and nuclear localization signal, features which are typical of transcription factors. AATF inhibits the ZIPK-mediated pro-apoptotic pathway and may activate other anti-apoptotic pathways. Recently, it has also been shown to protect neural cells against oxidative damage induced by amyloid b-peptide and to inhibit aberrant production of the beta-peptide by interacting with Par-4 (prostate apoptosis response-4), another pro-apoptotic leucine zipper protein that is associated with neuronal degeneration in Alzheimer's disease (AD), suggesting that AATF may have potential therapeutic applications in both familial and sporadic forms of AD.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A03945 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

AATF antibody can be used for the detection of AATF by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information AATF

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