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Product Info Summary
| SKU: | A01243 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-Protein ALEX GNAS Antibody
SKU/Catalog Number
A01243
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Protein ALEX GNAS Antibody catalog # A01243. Tested in WB,ICC/IF,IP applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Protein ALEX GNAS Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01243)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
A synthetic peptide of human GNAS(NP_000507.1).
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01243 is reactive to GNAS in Human, Mouse, Rat
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01243 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IP: 1:50-1:200
Validation Images & Assay Conditions
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Western blot analysis of extracts of various cell lines, using GNAS antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.
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Immunofluorescence analysis of L929 cells using GNAS Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U2OS cells using GNAS Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunoprecipitation analysis of 200ug extracts of Mouse brain cells using 3ug GNAS antibody . Western blot was performed from the immunoprecipitate using GNAS antibody at a dilution of 1:1000.
Specific Publications For Anti-Protein ALEX GNAS Antibody (A01243)
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