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Product Info Summary
| SKU: | A00513 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Protein delta homolog 1 DLK1 Antibody
SKU/Catalog Number
A00513
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Protein delta homolog 1 DLK1 Antibody (Catalog # A00513). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
DLK1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Protein delta homolog 1 DLK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00513)
Host
Rabbit
Contents
DLK1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
DLK1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human DLK1. The immunogen is located within amino acids 260 - 310 of DLK1.
Cross-reactivity
At least four isoforms of DLK1 are known to exist.
Reactive Species
A00513 is reactive to DLK1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
41.3 kDa
Background of DLK1
The Delta-like 1 homolog (DLK1) is a transmembrane protein containing six epidermal growth factor repeats. It is involved in the differentiation of several cell types, including adipocytes and is also thought to be a tumor suppressor. The DLK1 gene is one of several imprinted genes located in a region on chromosome 14q32; certain mutations in this imprinted region can cause phenotypes similar to maternal and paternal uniparental disomy of chromosome 14 (UPD14). DLK1 is expressed from the paternal allele; a polymorphism within this gene has been associated with child and adolescent obesity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00513 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
DLK1 antibody can be used for detection of DLK1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of HepG2 in 293 cell lysate with DLK1 antibody at (A) 1 and (B) 2 μg/mL.
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Immunocytochemistry of DLK1 in HepG2 cells with DLK1 antibody at 2.5 μg/ml.
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Immunofluorescence of DLK1 in HepG2 cells with DLK1 antibody at 20 μg/ml.
Specific Publications For Anti-Protein delta homolog 1 DLK1 Antibody (A00513)
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