Product Info Summary
SKU: | A02308-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Protein diaphanous homolog 1 DIAPH1 Antibody
SKU/Catalog Number
A02308-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Protein diaphanous homolog 1 DIAPH1 Antibody catalog # A02308-1. Tested in WB,ICC,IF,IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Protein diaphanous homolog 1 DIAPH1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02308-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Keratin 10.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02308-1 is reactive to DIAPH1 in Human
Applications
A02308-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
141.347kDa
Background of DIAPH1
This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21.
XM Zhou, J. Biol. Chem., Oct 1988; 263: 15584 - 15589.
Felix B. M¨¹ller, Hum. Mol. Genet., Apr 2006; 15: 1133 - 1141.
J Reichelt, J. Cell Sci., Sep 1997; 110: 2175.
BP Korge, PNAS, Feb 1992; 89: 910.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:5,000-1:10,000
ICC: 1:50
IHC: 1:50-1:100
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of DIAPH1 using Anti-Protein diaphanous homolog 1 DIAPH1 Antibody (A02308-1).
ICC staining DIAPH1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-Protein diaphanous homolog 1 DIAPH1 Antibody A02308-1
Western blot analysis of DIAPH1 on SiHa cell lysate using anti-DIAPH1 antibody at 1/5Protein Target Info & Infographic
Gene/Protein Information For DIAPH1 (Source: Uniprot.org, NCBI)
Gene Name
DIAPH1
Full Name
Protein diaphanous homolog 1
Weight
141.347kDa
Superfamily
formin homology family
Alternative Names
DIA1; diaphanous homolog 1 (Drosophila); hDIA1; homolog) 1; LFHL1FLJ25265 DIAPH1 DFNA1, DIA1, DRF1, LFHL1, SCBMS, hDIA1 diaphanous related formin 1 protein diaphanous homolog 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DIAPH1, check out the DIAPH1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DIAPH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Protein diaphanous homolog 1 DIAPH1 Antibody (A02308-1)
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No publications found for A02308-1
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