Anti-Protein Hook homolog 3 HOOK3 Antibody

Product Name

Anti-Protein Hook homolog 3 HOOK3 Antibody

View all HOOK3 Antibodies

SKU/Catalog Number

A07701

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Protein Hook homolog 3 HOOK3 Antibody (Catalog # A07701). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

HOOK3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-Protein Hook homolog 3 HOOK3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07701)

Host

Rabbit

Contents

HOOK3 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

HOOK3 antibody was raised against a 19 amino acid peptide from near the amino terminus of human HOOK3. The immunogen is located within amino acids 210 - 260 of HOOK3.

Cross-reactivity

HOOK3 antibody is human, mouse and rat reactive. HOOK3 antibody is predicted to not cross-react with HOOK1 and HOOK2.

Reactive Species

A07701 is reactive to HOOK3 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

83.1 kDa

Background of HOOK3

The HOOK proteins (HOOK1, HOOK2 and HOOK3) comprise a family of cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules mediating the spatial organization of diverse membrane-trafficking systems; their more divergent C-terminal domains mediate binding to organelles (1,2). HOOK3 exists as a homodimer and participates in defining the architecture and localization of the Golgi complex through its central coiled-coil domain (3). HOOK3 may regulate clearance of endocytosed receptors such as MSR1 (3,4).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A07701 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

HOOK3 antibody can be used for the detection of HOOK3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry at 10 μg/ml.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information HOOK3

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