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Product Info Summary
| SKU: | A00640 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Protein jagged-1 JAG1 Antibody
SKU/Catalog Number
A00640
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Protein jagged-1 JAG1 Antibody (Catalog # A00640). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Protein jagged-1 JAG1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00640)
Host
Rabbit
Contents
JAG1 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
JAG1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human JAG1. The immunogen is located within the last 50 amino acids of JAG1.
Cross-reactivity
JAG1 antibody is human, mouse and rat reactive. At least two isoforms of JAG1 are known to exist; this antibody will detect both isoforms.
Reactive Species
A00640 is reactive to JAG1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
133.8 kDa
Background of JAG1
The LIN-12/Notch family of transmembrane receptors plays a central role in development by regulating cell fate decisions (1). Ligands for Notch include JAG1, JAG2 and Delta. JAG1 is one of the DSL (Delta, Serrate, Lag2) transmembrane proteins that can activate Notch. It is involved in mammalian cardiovascular development and in cell-fate decisions during hematopoiesis (1,2). JAG1 is widely expressed in adult and fetal tissues and the mutation of the JAG1 gene is associated with Familial Tetrology of Fallot (3). JAG1 expression is associated with prostate cancer metastasis and is up-regulated in cervical squamous cell carcinoma (4,5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00640 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
JAG1 antibody can be used for detection of JAG1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of JAG1 in HeLa cell lysate with JAG1 antibody at 1 μg/ml.
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Immunohistochemistry of JAG1 in human spleen tissue with JAG1 antibody at 5 μg/ml.
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Immunofluorescence of JAG1 in human spleen tissue with JAG1 antibody at 20 μg/ml.
Specific Publications For Anti-Protein jagged-1 JAG1 Antibody (A00640)
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