Anti-Protein lin-54 homolog LIN54 Antibody

Product Name

Anti-Protein lin-54 homolog LIN54 Antibody

View all LIN54 Antibodies

SKU/Catalog Number

A09336

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Protein lin-54 homolog LIN54 Antibody (Catalog # A09336). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

LIN54 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-Protein lin-54 homolog LIN54 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09336)

Host

Rabbit

Contents

LIN54 antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

LIN54 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human LIN54. The immunogen is located within the last 50 amino acids of LIN54.

Cross-reactivity

LIN54 antibody is human, mouse, and rat reactive. Multiple isoforms of LIN54 are known to exist.

Reactive Species

A09336 is reactive to LIN54 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

79.5 kDa

Background of LIN54

LIN54 is a component of the LIN, or DREAM, complex, an essential regulator of cell cycle genes. This complex functions as a transcriptional repressor by binding to promoters of E2F target genes that regulate the G1/S transition (1). LIN54 is required for cell cycle progression and can bind to the cdc2 promoter in a sequence-specific manner (2).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A09336 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

LIN54 antibody can be used for detection of LIN54 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information LIN54

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