Product Info Summary
| SKU: | A32476 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-Protein NipSnap homolog 2 NIPSNAP2 Antibody
SKU/Catalog Number
A32476
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Protein NipSnap homolog 2 NIPSNAP2 Antibody (Catalog # A32476). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
NIPSNAP2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Protein NipSnap homolog 2 NIPSNAP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A32476)
Host
Rabbit
Contents
NIPSNAP2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
NIPSNAP2 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human NIPSNAP2. The immunogen is located within the first 50 amino acids of NIPSNAP2.
Reactive Species
A32476 is reactive to GBAS in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
33.7 kDa
Background of GBAS
The NIPSNAP proteins comprise a family of evolutionarily well-conserved proteins with strong sequence similarity to the central portion of a protein encoded by C. elegans chromosome III between a 4-nitrophenylphosphatase (NIP) domain and non-neuronal SNAP25-like protein. NIPSNAP2, a novel gene encoding a protein with tyrosine phosphorylation sites and a transmembrane domain, is co-amplified with EGFR in approximately 40% of glioblastomas, the most common and malignant form of central nervous system tumors. It is widely expressed and most abundant in heart and skeletal muscle. NIPSNAP proteins have been suggested to be important in vesicular transport. NIPSNAP2 antibody is predicted to not cross-react with any other members of the NIPSNAP protein family.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A32476 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
NIPSNAP2 antibody can be used for detection of NIPSNAP2 by Western blot at 0.5 and 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of NIPSNAP2 in human skeletal muscle tissue lysate with NIPSNAP2 antibody at (A) 0.5 and (B) 1 μg/mL.
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Immunohistochemistry of NIPSNAP2 in mouse skeletal muscle tissue with NIPSNAP2 antibody at 2.5μg/mL.
Specific Publications For Anti-Protein NipSnap homolog 2 NIPSNAP2 Antibody (A32476)
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