Product Info Summary
SKU: | A03465 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | IP, IHC, WB |
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Product info
Product Name
Anti-Protein O-GlcNAcase MGEA5 Antibody
View all O-GlcNAcase/OGA/MGEA5 Antibodies
SKU/Catalog Number
A03465
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Protein O-GlcNAcase MGEA5 Antibody catalog # A03465. Tested in WB,IHC,IP applications. This antibody reacts with Human,Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Protein O-GlcNAcase MGEA5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03465)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human FEV.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03465 is reactive to OGA in Human, Mouse
Applications
A03465 is guaranteed for IP, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
102.915kDa
Background of O-GlcNAcase/OGA/MGEA5
Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. May be involved in the remodeling of the actin cytoskeleton. Binds actin with high affinity both in the absence and presence of ATP and its mechanochemical activity is inhibited by calcium ions. Also acts as a GTPase activating protein on Rho.
Wirth J.A., J. Cell Sci. 109:653-661(1996).
Grewal P.K., Gene 240:389-398(1999).
Post P.L., J. Cell Sci. 111:941-950(1998).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2,000
IHC: 1:50-1:200
IP: 1:10-1:50
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of MGEA5 using Anti-Protein O-GlcNAcase MGEA5 Antibody (A03465).
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MGEA5 antibody. Counter stained with hematoxylin.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-Protein O-GlcNAcase MGEA5 Antibody A03465
Western blot analysis of MGEA5 on PC-3M and SH-SY-5Y cell lysates using anti-MGEA5 antibody at 1/500 dilution.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For OGA (Source: Uniprot.org, NCBI)
Gene Name
OGA
Full Name
Protein O-GlcNAcase
Weight
102.915kDa
Superfamily
glycosyl hydrolase 84 family
Alternative Names
bifunctional protein NCOAT; FLJ11229; GH84; HEXC; HEXC3; Hexosaminidase B; hyaluronidase in meningioma; KIAA0679; MEA5FLJ23355; meningioma expressed antigen 5 (hyaluronidase); Meningioma-expressed antigen 5; MGEA5; NCOAT; Nuclear cytoplasmic O-GlcNAcase and acetyltransferase; OGA; OGlcNAcase; O-GlcNAcase OGA MEA5, MGEA5, NCOAT O-GlcNAcase protein O-GlcNAcase|N-acetyl-beta-D-glucosaminidase|N-acetyl-beta-glucosaminidase|beta-N-acetylglucosaminidase|beta-N-acetylhexosaminidase|beta-hexosaminidase|bifunctional protein NCOAT|hyaluronidase in meningioma|meningioma expressed antigen 5 (hyaluronidase)|meningioma-expressed antigen 5|nuclear cytoplasmic O-GlcNAcase and acetyltransferase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on OGA, check out the OGA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for OGA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Protein O-GlcNAcase MGEA5 Antibody (A03465)
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No publications found for A03465
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