Anti-Protein shisa-4 SHISA4 Antibody

Product Name

Anti-Protein shisa-4 SHISA4 Antibody

View all SHISA4 Antibodies

SKU/Catalog Number

A16391

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Protein shisa-4 SHISA4 Antibody (Catalog # A16391). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

SHISA4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-Protein shisa-4 SHISA4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A16391)

Host

Rabbit

Contents

SHISA4 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

SHISA4 antibody was raised against a 15 amino acid peptide near the center of human SHISA4 . The immunogen is located within amino acids 100 - 150 of SHISA4.

Cross-reactivity

SHISA4 antibody is predicted to not cross-react with other SHISA protein family members.

Reactive Species

A16391 is reactive to SHISA4 in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

21.5 kDa

Background of SHISA4

SHISA4 plays an essential role in the maturation of presomitic mesoderm cells by individual attenuation of both FGF and WNT signaling. The Shisa family of single-transmembrane proteins is characterized by an N-terminal cysteine-rich domain and a proline-rich C-terminal region. Its founding member, Xenopus Shisa, promotes head development by antagonizing Wnt and FGF signaling. Shisa physically interacted with immature forms of the Wnt receptor Frizzled and the FGF receptor within the ER and inhibited their posttranslational maturation and trafficking to the cell surface. Loss of Shisa function sensitized the neuroectoderm to Wnt signaling and suppressed head formation during gastrulation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A16391 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

SHISA4 Antibody can be used for detection of SHISA4 by Western blot starting at 1 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information SHISA4

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis