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Product Info Summary
| SKU: | A12817 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Protein Shroom1 SHROOM1 Antibody
SKU/Catalog Number
A12817
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Protein Shroom1 SHROOM1 Antibody (Catalog # A12817). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
SHROOM1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Protein Shroom1 SHROOM1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12817)
Host
Rabbit
Contents
SHROOM1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Rabbit polyclonal SHROOM1 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human SHROOM1. The immunogen is located within the last 50 amino acids of SHROOM1.
Cross-reactivity
SHROOM1 antibody is human, mouse and rat reactive. At least three isoforms of SHROOM1 are known to exist; this antibody will detect only the two largest isoforms. SHROOM1 antibody is predicted to not cross-react with other SHROOM family members.
Reactive Species
A12817 is reactive to SHROOM1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
90.8 kDa
Background of SHROOM1
SHROOM family members play diverse roles in the development of the nervous system and other tissues. SHROOM1 is a PDZ domain-containing actin-binding protein that is required for neural tube morphogenesis. It facilitates neurulation by regulating the morphology of neurepithelial cells via the apical positioning of an actomyosin network in the neurepithelium. SHROOM1 is also expressed in pulmonary arterial smooth muscle cells and its expression is significantly decreased in mouse and human models of pulmonary arterial hypertension, suggesting that there may be a link between SHROOM1 expression and pulmonary arterial hypertension.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12817 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SHROOM1 antibody can be used for detection of SHROOM1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SHROOM1 in mouse heart tissue lysate with SHROOM1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Specific Publications For Anti-Protein Shroom1 SHROOM1 Antibody (A12817)
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