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Product Info Summary
| SKU: | A04911 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Protein Shroom3 SHROOM3 Antibody
SKU/Catalog Number
A04911
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Protein Shroom3 SHROOM3 Antibody (Catalog # A04911). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
SHROOM3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Protein Shroom3 SHROOM3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04911)
Host
Rabbit
Contents
SHROOM3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Rabbit polyclonal SHROOM3 antibody was raised against an 18 amino acid peptide near the center of human SHROOM3. The immunogen is located within amino acids 850 - 900 of SHROOM3.
Cross-reactivity
SHROOM3 antibody is human specific. At least four isoforms of SHROOM3 are known to exist; this antibody will detect all four isoforms. SHROOM3 antibody is predicted to not cross-react with other SHROOM family members.
Reactive Species
A04911 is reactive to SHROOM3 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
216.9 kDa
Background of SHROOM3
SHROOM family members play diverse roles in the development of the nervous system and other tissues. SHROOM3 is an actin-binding protein is a regulator of the microtubule cytoskeleton during epithelial morphogenesis and is sufficient and necessary to induce a redistribution of the microtubule regulator gamma-tubulin. It is necessary for both apiscobasal cell elongation and apical constriction in the neural epithelium. SHROOM3 has also been shown to be essential for cell shape changes and morphogenesis in the developing vertebrate gut, with its transcription relying on the Pitx1 transcription factor.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04911 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SHROOM3 antibody can be used for detection of SHROOM3 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SHROOM3 in SK-N-SH cell lysate with SHROOM3 antibody at 1 μg/mL.
Specific Publications For Anti-Protein Shroom3 SHROOM3 Antibody (A04911)
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