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Product Info Summary
| SKU: | A09477 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Protein TESPA1 TESPA1 Antibody
SKU/Catalog Number
A09477
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Protein TESPA1 TESPA1 Antibody (Catalog # A09477). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.
Storage & Handling
TESPA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Protein TESPA1 TESPA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09477)
Host
Rabbit
Contents
TESPA1 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
TESPA1 antibody was raised against an 18 amino acid peptide near the amino terminus of human TESPA1. The immunogen is located within amino acids 20 - 70 of TESPA1.
Cross-reactivity
TESPA1 antibody is human specific. At least three isoforms TESPA1 are known to exist.
Reactive Species
A09477 is reactive to TESPA1 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
59.2 kDa
Background of TESPA1
TESPA1 is a critical component of the TCR signalosome and is essential for T cell selection and maturation through the regulation of TCR signaling during T cell development (1). TESPA1 encodes a protein of 458 amino acids and is highly conserved among vertebrate species (2). TESPA1 expression was tightly regulated during T cell development, with highest expression at the DP stage (3). TESPA1 deficiency resulted in failure of positive selection and substantially fewer of CD4+ and CD8+ SP cells in the thymus (1,4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09477 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TESPA1 antibody can be used for detection of TESPA1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of TESPA1 in A431 cell lysate with TESPA1 antibody at 1 μg/ml.
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Immunocytochemistry of TESPA1 in A431 cells with TESPA1 antibody at 5 μg/mL.
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Immunofluorescence of TESPA1 in A431 cells with TESPA1 antibody at 20 μg/mL.
Specific Publications For Anti-Protein TESPA1 TESPA1 Antibody (A09477)
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