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Product Info Summary
| SKU: | A05994-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-Protein TMEPAI PMEPA1 Antibody
SKU/Catalog Number
A05994-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Protein TMEPAI PMEPA1 Antibody (Catalog # A05994-1). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
PMEPA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Protein TMEPAI PMEPA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05994-1)
Host
Rabbit
Contents
PMEPA1 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
PMEPA1 antibody was raised against a 16 amino acid peptide near the center of human PMEPA1. The immunogen is located within amino acids 160 - 210 of PMEPA1.
Cross-reactivity
PMEPA1 antibody is human, mouse and rat reactive. At least four isoforms are known to exist; this antibody will detect all of the isoforms.
Reactive Species
A05994-1 is reactive to PMEPA1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
31.6 kDa
Background of PMEPA1
The prostate transmembrane protein, androgen induced 1 (PMEPA1) protein is a transmembrane protein that contains a Smad interacting motif (SIM) (1,2). Expression of this gene is induced by androgens and transforming growth factor beta, and the encoded protein suppresses the androgen receptor and transforming growth factor beta signaling pathways though interactions with Smad proteins (3). Overexpression of this gene may play a role in multiple types of cancer (2,4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05994-1 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PMEPA1 antibody can be used for detection of PMEPA1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of PMEPA1 inA549 cell lysate with PMEPA1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of PMEPA1 in human prostate tissue with PMEPA1 antibody at 2.5 μg/ml.
Specific Publications For Anti-Protein TMEPAI PMEPA1 Antibody (A05994-1)
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