Anti-Protein Wiz WIZ Antibody

Product Name

Anti-Protein Wiz WIZ Antibody

View all WIZ Antibodies

SKU/Catalog Number

A05707

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Protein Wiz WIZ Antibody (Catalog # A05707). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

WIZ antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Protein Wiz WIZ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05707)

Host

Rabbit

Contents

WIZ Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

WIZ antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human TFF3. The immunogen is located within the last 50 amino acids of WIZ.

Cross-reactivity

At least four isoforms of WIZ are known to exist; this antibody will detect all four.

Reactive Species

A05707 is reactive to WIZ in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

178.7 kDa

Background of WIZ

WIZ, also known as ZNF803, was initially identified in a mouse cerebellum cDNA library screen and its message was found to be expressed in the granule cell layers of the cerebellum as well as in the dentate gyrus and olfactory bulb. Later analysis indicates however that WIZ is ubiquitously expressed. WIZ is a nuclear protein that co-localizes with G9a, a histone methyltransferase responsible for mono- and dimethylation of H3K9 at euchromatic regions. WIZ can also associate with CtBP family proteins, known to be transcriptional co-repressors, and has been suggested to link G9a/GLP complexes to the CtBP co-repressor machinery, possibly regulating complex stability and gene silencing.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A05707 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WIZ antibody can be used for detection of WIZ by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information WIZ

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