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Product Info Summary
| SKU: | A03479 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Protein Wnt-10a Wnt10a Antibody
SKU/Catalog Number
A03479
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Protein Wnt-10a Wnt10a Antibody (Catalog # A03479). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Wnt10a antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Protein Wnt-10a Wnt10a Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03479)
Host
Rabbit
Contents
Wnt10a Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Wnt10a antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Wnt10a. The immunogen is located within amino acids 300 - 350 of Wnt10a.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This Wnt10a antibody is predicted to not cross-react with Wnt10b.
Reactive Species
A03479 is reactive to Wnt10a in Human, Mouse, Rat
Applications
A03479 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
46.454kDa
Background of Wnt-10a
Wnt10a is a member of the Wnt family, a gene family that encodes secreted signaling proteins that play crucial roles in normal development such as regulation of cell fate and patterning during embryogenesis as well as neoplastic transformation. Elevated levels of Wnt10a have been detected in several human tumors and cancer cell lines including colorectal cancer and chronic lymphocytic leukemia and it has been suggested that uncontrolled Wnt signaling may contribute to the defect in apoptosis that characterizes these malignancies. Recently, a mutation in Wnt10a was found to be associated with odonto-onycho-dermal dysplasia, an autosomal recessive ectodermal dysplasia.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Wnt10a antibody can be used for detection of Wnt10a by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Wnt10a in RAW264.7 cell lysate with Wnt10a antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of Wnt10a in human skeletal muscle tissue with Wnt10a antibody at 10 μg/mL.
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Immunofluorescence of Wnt10a in Human Skeletal Muscle tissue with Wnt10a antibody at 20 μg/mL.
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Immunohistochemistry of Wnt10a in mouse skeletal muscle tissue with Wnt10a antibody at 5 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For Wnt10a (Source: Uniprot.org, NCBI)
Gene Name
Wnt10a
Full Name
Protein Wnt-10a
Weight
46.454kDa
Superfamily
Wnt family
Alternative Names
FLJ14301; protein Wnt-10a; SSPS; wingless-type MMTV integration site family, member 10A; Wnt10a; Wnt-10a Wnt10a|wingless-type MMTV integration site family, member 10A|protein Wnt-10a|wingless related MMTV integration site 10a
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Wnt10a, check out the Wnt10a Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Wnt10a: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Protein Wnt-10a Wnt10a Antibody (A03479)
Hello CJ!
A03479 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
An R,Wang X,Yang L,Zhang J,Wang N,Xu F,Hou Y,Zhang H,Zhang L. Polystyrene microplastics cause granulosa cells apoptosis and fibrosis in ovary through oxidative stress in rats.Toxicology.2020 Dec 24;449:152665. doi:10.1016/j.tox.2020.152665.Epub ahead of p
Species: Rat
A03479 usage in article: APP:WB, SAMPLE:OVARY TISSUE, DILUTION:1:500
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Customer Reviews
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