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Product Info Summary
| SKU: | A17179 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Protein yippee-like 4 YPEL4 Antibody
SKU/Catalog Number
A17179
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Protein yippee-like 4 YPEL4 Antibody (Catalog # A17179). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
YPEL4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Protein yippee-like 4 YPEL4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A17179)
Host
Rabbit
Contents
YPEL4 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
YPEL4 antibody was raised against a 12 amino acid synthetic peptide near the amino terminus of human YPEL4. The immunogen is located within the first 50 amino acids of YPEL4.
Reactive Species
A17179 is reactive to YPEL4 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
14.3 kDa
Background of YPEL4
YPEL4 (yippee-like 4) belongs to a family of five yippee-like proteins, all of which localize to the centrosome or mitotic spindle and are widely expressed in both adult and fetal tissue. This localization plus the fact that the family of human YPEL proteins share a high degree of sequence homology across species suggests that these proteins may have a conserved function involved in cell division. YPEL4 is ubiquitously expressed in adult tissues and has been shown to associate with the major vault protein (MVP). It has been suggested that MVP can inhibit YPEL4's ability to activate Elk-1 in the MAPK signaling pathway.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A17179 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
YPEL4 antibody can be used for detection of YPEL4 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of YPEL4 in SW480 cell lysate with YPEL4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunocytochemistry of YPEL4 in SW480 cells with YPEL4 antibody at 2.5 μg/ml.
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Immunofluorescence of YPEL4 in SW480 cells with YPEL4 antibody at 5 μg/ml.
Specific Publications For Anti-Protein yippee-like 4 YPEL4 Antibody (A17179)
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