Anti-Prothrombin Antibody Picoband™ (monoclonal, 4G13G9)

Product Name

Anti-Prothrombin Antibody Picoband™ (monoclonal, 4G13G9)

View all Coagulation Factor II/Thrombin Antibodies

SKU/Catalog Number

M00044-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Prothrombin Antibody Picoband™ (monoclonal, 4G13G9) catalog # M00044-2. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Prothrombin Antibody Picoband™ (monoclonal, 4G13G9) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00044-2)

Host

Mouse

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Monoclonal

Clone Number

4G13G9

Isotype

Mouse IgG2b

Immunogen

E. coli-derived human Prothrombin recombinant protein (Position: Y97-R124).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M00044-2 is reactive to F2 in Human

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Observed Molecular Weight

70 kDa

Calculated molecular weight

14377 MW

Background of Coagulation Factor II/Thrombin

F2 (Coagulation Factor II), also known as thrombin, is a serine protease that in humans is encoded by the F2 gene. This gene for human prothrombin (F2) was assigned to chromosome 11p11-q12 by analysis of a panel of somatic cell hybrid DNAs and by in situ hybridization, using both cDNA and genomic probes. The activated thrombin enzyme plays an important role in hemostasis and thrombosis: it converts fibrinogen to fibrin for blood clot formation, stimulates platelet aggregation, and activates coagulation factors V, VIII (F8), and XIII (F13A1). Thrombin also inhibits coagulation by activating protein C.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00044-2 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human

Positive Control

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Prothrombin using anti-Prothrombin antibody (M00044-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Prothrombin antigen affinity purified monoclonal antibody (Catalog # M00044-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Prothrombin at approximately 70 kDa. The expected band size for Prothrombin is at 70 kDa.

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Figure 2. IHC analysis of Prothrombin using anti-Prothrombin antibody (M00044-2).
Prothrombin was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Prothrombin Antibody (M00044-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

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Figure 3. IHC analysis of Prothrombin using anti-Prothrombin antibody (M00044-2).
Prothrombin was detected in a paraffin-embedded section of human colorectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Prothrombin Antibody (M00044-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

Click image to see more details

Figure 4. IHC analysis of Prothrombin using anti-Prothrombin antibody (M00044-2).
Prothrombin was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Prothrombin Antibody (M00044-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

Gene/Protein Information For F2 (Source: Uniprot.org, NCBI)

Alternative Names

coagulation factor II (thrombin) receptor-like 2; Coagulation factor II receptor-like 2 (protease-actovated receptor 3); Coagulation factor II receptor-like 2; Coagulation Factor II; F2; PAR-3; PAR3proteinase-activated receptor 3; protease-activated receptor 3; proteinase-activated receptor-3; PT; serine protease; Thrombin receptor-like 2; Thrombin F2 PT, RPRGL2, THPH1 coagulation factor II, thrombin prothrombin|prepro-coagulation factor II|prothrombin B-chain|thrombin factor II

For more info on F2, check out the F2 Infographic

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In this infographic, you will see the following information for F2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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