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Product Info Summary
| SKU: | M01985-2 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PROX1 Antibody (Center)
SKU/Catalog Number
M01985-2
Size
80 µl
Description
Boster Bio Anti-PROX1 Antibody (Center) (Catalog # M01985-2). Tested in WB application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-PROX1 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01985-2)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This PROX1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 444-478 amino acids from the Central region of human PROX1.
Reactive Species
M01985-2 is reactive to PROX1 in Human, Mouse
Calculated molecular weight
83.2 kDa
Background of PROX1
Transcription factor involved in developmental processes such as cell fate determination, gene transcriptional regulation and progenitor cell regulation in a number of organs. Plays a critical role in embryonic development and functions as a key regulatory protein in neurogenesis and the development of the heart, eye lens, liver, pancreas and the lymphatic system. Involved in the regulation of the circadian rhythm. Represses: transcription of the retinoid-related orphan receptor RORG, transcriptional activator activity of RORA and RORG and the expression of RORA/G-target genes including core clock components: ARNTL/BMAL1, NPAS2 and CRY1 and metabolic genes: AVPR1A and ELOVL3.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01985-2 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
Validation Images & Assay Conditions
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All lanes : Anti-PROX1 Antibody (Center) at 1:1000 dilution
Lane 1: human heart lysates
Lane 2: mouse brain lysates
Lane 3: HepG2 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 83 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Anti-PROX1 Antibody (Center) at 1:2000 dilution + SH-SY5Y whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 83 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-PROX1 Antibody (Center) (M01985-2)
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