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Product Info Summary
| SKU: | A02846-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-PSMA/GCPII/FOLH1 Antibody Picoband®
SKU/Catalog Number
A02846-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PSMA/GCPII/FOLH1 Antibody Picoband® catalog # A02846-2. Tested in WB, IHC, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PSMA/GCPII/FOLH1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02846-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human PSMA/GCPII/FOLH1 recombinant protein (Position: K55-A750).
Reactive Species
A02846-2 is reactive to FOLH1 in Human, Mouse, Rat
Observed Molecular Weight
100 kDa
Calculated molecular weight
84.3 kDa
Background of FOLH1
This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants encoding several different isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02846-2 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 0.25-0.5 μg/ml | Human |
| Immunohistochemistry(Paraffin-embedded Section) | 2-5 μg/ml | Mouse, Rat |
| Flow Cytometry (Fixed) | 1-3 μg/1x106 cells | Human |
| ELISA | 0.1-0.5 μg/ml |
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Validation Images & Assay Conditions
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Western blot analysis of PSMA/GCPII/FOLH1 using anti-PSMA/GCPII/FOLH1 antibody (A02846-2).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human LNCAP whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMA/GCPII/FOLH1 antigen affinity purified polyclonal antibody (A02846-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PSMA/GCPII/FOLH1 at approximately 100 kDa. The expected band size for PSMA/GCPII/FOLH1 is at 84 kDa.
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IHC analysis of PSMA/GCPII/FOLH1 using anti-PSMA/GCPII/FOLH1 antibody (A02846-2).
PSMA/GCPII/FOLH1 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMA/GCPII/FOLH1 Antibody (A02846-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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IHC analysis of PSMA/GCPII/FOLH1 using anti-PSMA/GCPII/FOLH1 antibody (A02846-2).
PSMA/GCPII/FOLH1 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMA/GCPII/FOLH1 Antibody (A02846-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Flow Cytometry analysis of SH-SY5Y cells using anti-PSMA/GCPII/FOLH1 antibody (A02846-2).
Overlay histogram showing SH-SY5Y cells stained with A02846-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PSMA/GCPII/FOLH1 Antibody (A02846-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-PSMA/GCPII/FOLH1 Antibody Picoband® (A02846-2)
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