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Product Info Summary
| SKU: | M07499 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, IHC-P, WB |
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Product info
Product Name
Anti-PSMA5 Antibody
View all Proteasome 20S alpha 5 Antibodies
SKU/Catalog Number
M07499
Size
80 µl
Description
Boster Bio Anti-PSMA5 Antibody (Catalog # M07499). Tested in WB, IF, IHC-P application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-PSMA5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07499)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
426CT8.5.1
Isotype
IgG1
Immunogen
Purified His-tagged PSMA5 protein (Fragment) was used to produced this monoclonal antibody.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M07499 is reactive to PSMA5 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
26411 Da
Background of Proteasome 20S alpha 5
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M07499 is guaranteed for IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:1000
IHC-P: 1:25
Validation Images & Assay Conditions
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Fluorescent image of Hela cells stained with XAF1 PSMA5 Antibody. M07499 was diluted at 1:25 dilution. An Alexa Fluor® 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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PSMA5 Antibody (Cat. #M07499) western blot analysis in K562 cell line lysates (35μg/lane). This demonstrates the PSMA5 antibody detected the PSMA5 protein (arrow).
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All lanes : Anti-PSMA5 Antibody at 1:1000 dilution
Lane 1: human liver lysate
Lane 2: Hela whole cell lysate
Lane 3: HepG2 whole cell lysate
Lane 4: MCF-7 whole cell lysate
Lane 5: Neuro-2a whole cell lysate
Lane 6: PC-12 whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 26 kDa.
Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded H. testis section using PSMA5 Antibody (Cat#M07499). M07499 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Protein Target Info & Infographic
Gene/Protein Information For PSMA5 (Source: Uniprot.org, NCBI)
Gene Name
PSMA5
Full Name
Proteasome subunit alpha type-5
Weight
26411 Da
Superfamily
peptidase T1A family
Alternative Names
Proteasome subunit alpha type-5, Macropain zeta chain, Multicatalytic endopeptidase complex zeta chain, Proteasome zeta chain, PSMA5 PSMA5 PSC5, ZETA proteasome 20S subunit alpha 5 proteasome subunit alpha type-5|epididymis tissue sperm binding protein Li 11n|macropain subunit zeta|macropain zeta chain|multicatalytic endopeptidase complex zeta chain|proteasome (prosome, macropain) subunit, alpha type, 5|proteasome alpha 5 subunit|proteasome component 5|proteasome subunit alpha 5|proteasome subunit zeta|proteasome zeta chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PSMA5, check out the PSMA5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PSMA5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PSMA5 Antibody (M07499)
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