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Product Info Summary
| SKU: | A15227-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-PSMAL Antibody (Center)
SKU/Catalog Number
A15227-1
Size
80 µl
Description
Boster Bio Anti-PSMAL Antibody (Center) (Catalog # A15227-1). Tested in IHC-P, WB, Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-PSMAL Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15227-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This PSMAL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 155-183 amino acids from the Central region of human PSMAL.
Reactive Species
A15227-1 is reactive to FOLH1B in Human
Calculated molecular weight
50.0 kDa
Background of FOLH1B
PSMAL has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity (By similarity). PSMAL exhibits a dipeptidyl-peptidase IV type activity
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15227-1 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:50-1:100
FC: 1:10-1:50
Validation Images & Assay Conditions
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Western blot analysis of lysate from human kidney tissue lysate, using PSMAL Antibody (Center). A15227-1 was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.
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Western blot analysis of PSMAL Antibody (Center) in K562 cell line lysates (35ug/lane).PSMAL (arrow) was detected using the purified Pab.
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Immunohistochemical analysis of paraffin-embedded H. liver section using PSMAL Antibody (Center). A15227-1 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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PSMAL Antibody (Center) IHC analysis in formalin fixed and paraffin embedded hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PSMAL Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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PSMAL Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Specific Publications For Anti-PSMAL Antibody (Center) (A15227-1)
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