Anti-PSMD3 Antibody (C-Term)

Product Name

Anti-PSMD3 Antibody (C-Term)

View all Proteasome 19S S3 Antibodies

SKU/Catalog Number

M08767

Size

50 µl

Description

Boster Bio Anti-PSMD3 Antibody (C-Term) (Catalog # M08767). Tested in WB, Flow Cytometry, IHC-P application(s). This antibody reacts with Human, Mouse.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-PSMD3 Antibody (C-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08767)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Clone Number

1503CT789.209.250.52

Isotype

Rabbit IgG

Immunogen

This PSMD3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 482-515 amino acids from human PSMD3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M08767 is reactive to PSMD3 in Human, Mouse

Applications

M08767 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

60.978kDa

Background of Proteasome 19S S3

Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:2000
IHC-P: 1:250
FC: 1:25

Validation Images & Assay Conditions

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All lanes : Anti-PSMD3 Antibody (C-Term) at 1:2000 dilution
Lane 1: Jurkat whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: HepG2 whole cell lysate
Lane 4: MCF-7 whole cell lysate
Lane 5: NIH/3T3 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 61 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

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M08767 staining PSMD3 in human brain tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/250) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

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M08767 staining PSMD3 in human testis tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/250) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

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Overlay histogram showing HeLa cells stained with M08767 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M08767, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Gene/Protein Information For PSMD3 (Source: Uniprot.org, NCBI)

Alternative Names

26S proteasome regulatory subunit RPN3; 26S proteasome regulatory subunit S3; P58; proteasome (prosome, macropain) 26S subunit, non-ATPase, 3; Proteasome subunit p58; Rpn3; S3, 26S proteasome non-ATPase regulatory subunit 3; tissue specific transplantation antigen 2; TSTA2 PSMD3 P58, RPN3, S3, TSTA2 proteasome 26S subunit, non-ATPase 3 26S proteasome non-ATPase regulatory subunit 3|26S proteasome regulatory subunit RPN3|26S proteasome regulatory subunit S3|proteasome (prosome, macropain) 26S subunit, non-ATPase, 3|proteasome subunit p58|tissue specific transplantation antigen 2

For more info on PSMD3, check out the PSMD3 Infographic

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In this infographic, you will see the following information for PSMD3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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