Anti-PTEN Antibody (N-term)

PTEN antibody

Boster Bio Anti-PTEN Antibody (N-term) (Catalog # M00006-4). Tested in WB application(s). This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M00006-4
Size: 50 µl
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: WB

Customers Who Bought This Also Bought

Product Name

Anti-PTEN Antibody (N-term)

View all PTEN Antibodies

SKU/Catalog Number

M00006-4

Size

50 µl

Description

Boster Bio Anti-PTEN Antibody (N-term) (Catalog # M00006-4). Tested in WB application(s). This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-PTEN Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00006-4)

Host

Mouse

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Monoclonal

Clone Number

1096CT4.3.3

Isotype

IgG1,k

Immunogen

This PTEN antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 10-44 amino acids from the N-terminal region of human PTEN.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00006-4 is reactive to PTEN in Human, Mouse, Rat

Reconstitution

Calculated molecular weight

47166 Da

Background of PTEN

Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine- phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3- phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K- AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form induces less tumor suppressive ability. In motile cells, suppresses the formation of lateral pseudopods and thereby promotes cell polarization and directed movement.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

M00006-4 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For PTEN (Source: Uniprot.org, NCBI)

Gene Name

PTEN

Full Name

Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN

Weight

47166 Da

Superfamily

PTEN phosphatase protein family

Alternative Names

Phosphatidylinositol 3,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, 3.1.3.16, 3.1.3.48, 3.1.3.67, Mutated in multiple advanced cancers 1, Phosphatase and tensin homolog, PTEN, MMAC1, TEP1 PTEN 10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC11, PTENbeta, TEP1, PTEN phosphatase and tensin homolog phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN|MMAC1 phosphatase and tensin homolog deleted on chromosome 10|PTENepsilon|mitochondrial PTENalpha|mitochondrial phosphatase and tensin protein alpha|mutated in multiple advanced cancers 1|phosphatase and tensin-like protein|phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN|protein tyrosine phosphatase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PTEN, check out the PTEN Infographic

PTEN infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PTEN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for M00006-4

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-PTEN Antibody (N-term)?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

0 Reviews For Anti-PTEN Antibody (N-term)

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

0 Customer Q&As for Anti-PTEN Antibody (N-term)

Order DetailsPrice
M00006-4-50ul

50 μl

$299
M00006-4-200ul

200 μl

$347

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
In stock
Order Product
M00006-4
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.