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- Table of Contents
PTEN antibody (M00006-2) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Immunofluorescent analysis of Hela cells, using PTEN Antibody.
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Anti-PTEN Rabbit Monoclonal Antibody
PTEN antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC, ICC, WB |
Tested Applications
PTEN antibody was positively detected by WB in:
human Hela whole cell, human HepG2 whole cell, rat brain tissue, rat PC-12 whole cell, mouse brain tissue, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
PTEN antibody was positively detected by IHC in:
human colon tissue, Rat lung tissue, Human colon cancer tissue, Human tongue cancer tissue, Human liver tissue, Mouse skin tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
PTEN antibody was positively detected by ICC/IF in:
Hela cell
Recommend Dilution
Application
Dilution
Species
WB 1:1000-2000
IHC 1:50-200
ICC/IF 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Product Information
Product Name
Anti-PTEN Rabbit Monoclonal Antibody
View all PTEN Antibodies
View all PTEN Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
HBH-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PTEN
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
54 kDa
Calculated Molecular Weight
47.2 kDa
Cite This Product
Anti-PTEN Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00006-2)
Protocols
Western Blot protocol for PTEN antibody
Immunohistochemistry (IHC) protocol for PTEN antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for PTEN antibody
Publications
Application:
Species:
Oxidized DJ-1 activates the p-IKK/NF-κB/Beclin1 pathway by binding PTEN to induce autophagy and exacerbate myocardial ischemia-reperfusion injury
Published: 2024-03-18
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: WB
Impact Factor: 4.20
Species: Rat
PMID: 38508437
Review
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1 Review For Anti-PTEN Rabbit Monoclonal Antibody
This antibody is highly efficient and specific, suitable for Western blot detection of PTEN protein in rat colon tissue, with only minor nonspecific bands.
| SKU | M00006-2 |
|---|---|
| Application | Western Blot |
| Sample | rat colon tissue |
| Sample Processing Description | RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE. |
| Other Reagents | Blocking buffer |
| Primary Antibody | PTEN Rabbit Monoclonal Antibody |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Secondary Antibody | HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) |
| Secondary Incubation | 1:5000, 1 hour in room temperature |
| Detection | Substrate: ECL, Imaging system:ChemiDoc MP |
| Results Summary | The figure shows a schematic of Western blot results for the target protein PTEN and the internal control Actin in rat colon across different groups. Expression was increased in the model group. Among the low, medium, and high doses of the herbal treatment, the low-dose group showed the best effect. The target bands are clear, and the experimental results are satisfactory. |
Shiyu Zhang, LUTCM
Verified customer
Submitted 2026-01-06
Product Promise
We promise Anti-PTEN Rabbit Monoclonal Antibody performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
M00006-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026