Anti-PTGIS/PGIS Antibody Picoband™

Product Name

Anti-PTGIS/PGIS Antibody Picoband™

View all Prostaglandin I2 Synthase Antibodies

SKU/Catalog Number

A05053-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PTGIS/PGIS Antibody Picoband™ catalog # A05053-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PTGIS/PGIS Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05053-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PTGIS/PGIS recombinant protein (Position: D119-D470).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A05053-2 is reactive to PTGIS in Human, Mouse, Rat

Applications

A05053-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

57 kDa

Calculated molecular weight

57.104kDa

Background of Prostaglandin I2 Synthase

Prostaglandin-I synthase (EC 5.3.99.4) also known as prostaglandin I2 (prostacyclin) synthase (PTGIS) or CYP8A1 is an enzyme involved in prostanoid biosynthesis that in humans is encoded by the PTGIS gene. This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. However, this protein is considered a member of the cytochrome P450 superfamily on the basis of sequence similarity rather than functional similarity. This endoplasmic reticulum membrane protein catalyzes the conversion of prostglandin H2 to prostacyclin (prostaglandin I2), a potent vasodilator and inhibitor of platelet aggregation. An imbalance of prostacyclin and its physiological antagonist thromboxane A2 contribute to the development of myocardial infarction, stroke, and atherosclerosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PTGIS/PGIS using anti-PTGIS/PGIS antibody (A05053-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human SK-OV-3 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human SiHa whole cell lysates,
Lane 5: rat ovary tissue lysates,
Lane 6: rat heart tissue lysates,
Lane 7: mouse ovary tissue lysates,
Lane 8: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGIS/PGIS antigen affinity purified polyclonal antibody (Catalog # A05053-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGIS/PGIS at approximately 57 kDa. The expected band size for PTGIS/PGIS is at 57 kDa.

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Figure 2. Flow Cytometry analysis of U251 cells using anti-PTGIS/PGIS antibody (A05053-2).
Overlay histogram showing U251 cells stained with A05053-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTGIS/PGIS Antibody (A05053-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For PTGIS (Source: Uniprot.org, NCBI)

Alternative Names

CYP8; CYP8A1; CYP8A1PTGI; CYP8prostacyclin synthase; EC 5.3.99.4; MGC126858; MGC126860; PGIS; PGIScytochrome P450, family 8, subfamily A, polypeptide 1; Prostacyclin Synthase; prostaglandin I2 (prostacyclin) synthase; Prostaglandin I2 synthase; PTGI; PTGIS PTGIS CYP8, CYP8A1, PGIS, PTGI prostaglandin I2 synthase prostacyclin synthase|cytochrome P450, family 8, subfamily A, polypeptide 1|hydroperoxy icosatetraenoate dehydratase|prostaglandin I2 (prostacyclin) synthase

For more info on PTGIS, check out the PTGIS Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PTGIS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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