Anti-PTH2R Antibody Picoband™

Product Name

Anti-PTH2R Antibody Picoband™

View all PTH2R Antibodies

SKU/Catalog Number

A10343-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-PTH2R Antibody Picoband™ catalog # A10343-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PTH2R Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10343-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PTH2R recombinant protein (Position: A22-E511).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A10343-1 is reactive to PTH2R in Human

Applications

A10343-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

75 kDa

Calculated molecular weight

62.236kDa

Background of PTH2R

Parathyroid hormone 2 receptor is a protein that in humans is encoded by the PTH2R gene. The protein encoded by this gene is a member of the G-protein coupled receptor 2 family. This protein is a receptor for parathyroid hormone (PTH). This receptor is more selective in ligand recognition and has a more specific tissue distribution compared to parathyroid hormone receptor 1 (PTHR1). It is activated only by PTH and not by parathyroid hormone-like hormone (PTHLH) and is particularly abundant in brain and pancreas. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PTH2R using anti-PTH2R antibody (A10343-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTH2R antigen affinity purified polyclonal antibody (Catalog # A10343-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTH2R at approximately 75 kDa. The expected band size for PTH2R is at 62 kDa.

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Figure 2. IF analysis of PTH2R using anti-PTH2R antibody (A10343-1).
PTH2R was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PTH2R Antibody (A10343-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For PTH2R (Source: Uniprot.org, NCBI)

Alternative Names

parathyroid hormone 2 receptor; PTH2 receptor; PTH2R; PTHR2; PTHR2parathyroid hormone receptor 2 PTH2R PTHR2 parathyroid hormone 2 receptor parathyroid hormone 2 receptor|PTH2 receptor|parathyroid hormone receptor 2

For more info on PTH2R, check out the PTH2R Infographic

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In this infographic, you will see the following information for PTH2R: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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