Anti-PTPN22 Antibody Picoband™

Product Name

Anti-PTPN22 Antibody Picoband™

View all Lyp/PTPN22 Antibodies

SKU/Catalog Number

A00581-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PTPN22 Antibody Picoband™ catalog # A00581-3. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PTPN22 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00581-3)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PTPN22 recombinant protein (Position: M1-K42).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00581-3 is reactive to Ptpn22 in Human, Rat

Applications

A00581-3 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

92 kDa

Calculated molecular weight

89.714kDa

Background of Lyp/PTPN22

Protein tyrosine phosphatase, non-receptor type 22 (lymphoid), also known as PTPN22, is a protein that in humans is encoded by the PTPN22 gene. It is mapped to 1p13.2. This gene encodes of member of the non-receptor class 4 subfamily of the protein-tyrosine phosphatase family. The encoded protein is a lymphoid-specific intracellular phosphatase that associates with the molecular adapter protein CBL and may be involved in regulating CBL function in the T-cell receptor signaling pathway. Mutations in this gene may be associated with a range of autoimmune disorders including Type 1 Diabetes, rheumatoid arthritis, systemic lupus erythematosus and Graves' disease. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PTPN22 using anti-PTPN22 antibody (A00581-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human HL-60 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: rat PC-12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPN22 antigen affinity purified polyclonal antibody (Catalog # A00581-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTPN22 at approximately 92 kDa. The expected band size for PTPN22 is at 92 kDa.

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Figure 2. IF analysis of PTPN22 using anti-PTPN22 antibody (A00581-3).
PTPN22 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PTPN22 Antibody (A00581-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For Ptpn22 (Source: Uniprot.org, NCBI)

Alternative Names

EC 3.1.3.48; Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP; Lymphoid phosphatase; lymphoid-specific protein tyrosine phosphatase; Lyp; Lyp1; Lyp2; LyPTP; PEP; PEST-domain phosphatase; protein tyrosine phosphatase, non-receptor type 22 (lymphoid); protein tyrosine phosphatase, non-receptor type 8; PTPN22; PTPN8 (former); PTPN8LYP1; tyrosine-protein phosphatase non-receptor type 22 Ptpn22|70zp, 70zpep, PEP, Pt, Ptpn8|protein tyrosine phosphatase, non-receptor type 22 (lymphoid)|tyrosine-protein phosphatase non-receptor type 22|PEST domain-enriched tyrosine phosphatase|hematopoietic cell protein-tyrosine phosphatase 70Z-PEP|protein tyrosine phosphatase, non-receptor type 8

For more info on Ptpn22, check out the Ptpn22 Infographic

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In this infographic, you will see the following information for Ptpn22: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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