This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
CAVIN1 antibody (A31732-2) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
Filter:
Flow Cytometry analysis of SiHa cells using anti-PTRF/CAVIN1 antibody (A31732-2).
Overlay histogram showing SiHa cells stained with A31732-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTRF/CAVIN1 Antibody (A31732-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing SiHa cells stained with A31732-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTRF/CAVIN1 Antibody (A31732-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Bundle Save
Anti-PTRF/CAVIN1 Antibody Picoband®
CAVIN1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
CAVIN1 antibody was positively detected by WB in:
human Hela whole cell, human A549 whole cell, rat heart tissue, rat lung tissue, mouse heart tissue, mouse lung tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CAVIN1 antibody was positively detected by IHC in:
human glioblastoma tissue, human thyroid cancer tissue, human placenta tissue, human breast cancer tissue, human rectum adenocarcinoma tissue, human testicular seminoma tissue, human liver cancer tissue, human adrenocortical adenoma tissue, human spleen tissue, mouse lung tissue, rat lung tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
CAVIN1 antibody was positively detected by ICC/IF in:
Hela cell
CAVIN1 antibody was positively detected by FC in:
SiHa cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.25 μg/ml
Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
1-2 μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-PTRF/CAVIN1 Antibody Picoband®
View all CAVIN1 Antibodies
View all CAVIN1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PTRF/CAVIN1 recombinant protein (Position: D47-D390).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
50-55 kDa
Calculated Molecular Weight
43.5 kDa
Background of CAVIN1
This gene encodes a protein that enables the dissociation of paused ternary polymerase I transcription complexes from the 3' end of pre-rRNA transcripts. This protein regulates rRNA transcription by promoting the dissociation of transcription complexes and the reinitiation of polymerase I on nascent rRNA transcripts. This protein also localizes to caveolae at the plasma membrane and is thought to play a critical role in the formation of caveolae and the stabilization of caveolins. This protein translocates from caveolae to the cytoplasm after insulin stimulation. Caveolae contain truncated forms of this protein and may be the site of phosphorylation-dependent proteolysis. This protein is also thought to modify lipid metabolism and insulin-regulated gene expression. Mutations in this gene result in a disorder characterized by generalized lipodystrophy and muscular dystrophy.
Cite This Product
Anti-PTRF/CAVIN1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A31732-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
99%
High Homology
Dog
97%
High Homology
Rat
97%
High Homology
Mouse
96%
High Homology
Sheep
96%
High Homology
Zebrafish
66%
Low homology
Rabbit
39%
Low homology
Cow
36%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CAVIN1 antibody
Immunohistochemistry (IHC) protocol for CAVIN1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for CAVIN1 antibody
Flow Cytometry (FC) protocol for CAVIN1 antibody
Review
Have you used Anti-PTRF/CAVIN1 Antibody Picoband®?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
1 Review For Anti-PTRF/CAVIN1 Antibody Picoband®
Our lab will continue to use this antibody, and we will recommend it to other researchers at the university working on similar studies.
| SKU | A31732-2 |
|---|---|
| Application | Immunofluorescence |
| Sample | Normal rat liver and alcoholic rat liver |
| Sample Processing Description | The samples consist of myocardium from normal SD rats and myocardium from SD rats subjected to an alcoholic liver disease model. The alcoholic liver disease model was established by gavaging 50% ethanol twice daily, while allowing the rats to freely consume alcoholic beverages, for a total of 14 weeks. |
| Other Reagents | Goat Serum, DAPI, Anti-fade mounting medium |
| Primary Antibody | Anti-PTRF/CAVIN1 Antibody Picoband® |
| Primary Incubation | 1:200, overnight at 4 ℃ |
| Secondary Antibody | DyLight 488 Goat Anti-Rabbit IgG (H+L) |
| Secondary Incubation | 1:500, 45 minutes in room temperature |
| Detection | Imaging system:Leica DM2500 |
| Results Summary | PTRF/CAVIN1 is a multifunctional protein that shuttles between the plasma membrane and the nucleus, assisting in the recruitment of repair proteins when the plasma membrane is damaged. The results of this IF experiment indicate that alcohol gavage induces myocardial injury and leads to increased expression of PTRF. |
Yongqiu Zhen, Wannan Medical College
Verified customer
Submitted 2025-11-28
Product Promise
We promise Anti-PTRF/CAVIN1 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A31732-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026