Anti-PUMA/BBC3 Antibody Picoband™

PUMA antibody

Boster Bio Anti-PUMA/BBC3 Antibody Picoband™ catalog # A04899-3. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A04899-3
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-PUMA/BBC3 Antibody Picoband™

View all PUMA Antibodies

SKU/Catalog Number

A04899-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PUMA/BBC3 Antibody Picoband™ catalog # A04899-3. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PUMA/BBC3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04899-3)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence of human PUMA/BBC3 (EQWAREIGAQLRRMADDLNAQYE).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04899-3 is reactive to BBC3 in Human

Applications

A04899-3 is guaranteed for WB Boster Guarantee

Background of PUMA

The p53 upregulated modulator of apoptosis, or PUMA, is a pro-apoptotic member of the Bcl-2 protein family. The PUMA gene is located at 19q. PUMA transcript is contained within 4 exons, with the presumptive initiation codon in exon 2. The predicted 193-amino acid PUMA protein shares 91% amino acid identity with the murine sequence. Bcl-2 family members can form hetero- or homodimers, and they act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The expression of PUMA is regulated by the tumor suppressor p53, and PUMA has been shown to be involved in p53-mediated apoptosis. Additionally, PUMA encodes 2 BH3 domain-containing proteins, PUMA-alpha and PUMA-beta, that are produced through the use of an alternative first exon and are induced in cells following p53 activation. Furthermore, PUMA couples the nuclear and cytoplasmic proapoptotic functions of p53.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For BBC3 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9BXH1

Gene ID

27113

Gene Name

BBC3

Full Name

Bcl-2-binding component 3, isoforms 1/2

Weight

20.532kDa

Superfamily

Bcl-2 family

Alternative Names

BCL2 binding component 3; bcl-2-binding component 3; JFY1; JFY-1; p53 up-regulated modulator of apoptosis; PUMAFLJ42994 BBC3 JFY-1, JFY1, PUMA BCL2 binding component 3 bcl-2-binding component 3|p53 up-regulated modulator of apoptosis|p53-upregulated modulator of apoptosis

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on BBC3, check out the BBC3 Infographic

BBC3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for BBC3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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Size

Conjugation

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Total: $315

Size:100 μg

SKU:A04899-3

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A04899-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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