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Anti-PUMA/BBC3 Antibody Picoband™
Boster Bio Anti-PUMA/BBC3 Antibody Picoband™ catalog # A04899-3. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PUMA/BBC3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04899-3)
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
A synthetic peptide corresponding to a sequence of human PUMA/BBC3 (EQWAREIGAQLRRMADDLNAQYE).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
A04899-3 is reactive to BBC3 in Human
A04899-3 is guaranteed for WB Boster Guarantee
Background of PUMA
The p53 upregulated modulator of apoptosis, or PUMA, is a pro-apoptotic member of the Bcl-2 protein family. The PUMA gene is located at 19q. PUMA transcript is contained within 4 exons, with the presumptive initiation codon in exon 2. The predicted 193-amino acid PUMA protein shares 91% amino acid identity with the murine sequence. Bcl-2 family members can form hetero- or homodimers, and they act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The expression of PUMA is regulated by the tumor suppressor p53, and PUMA has been shown to be involved in p53-mediated apoptosis. Additionally, PUMA encodes 2 BH3 domain-containing proteins, PUMA-alpha and PUMA-beta, that are produced through the use of an alternative first exon and are induced in cells following p53 activation. Furthermore, PUMA couples the nuclear and cytoplasmic proapoptotic functions of p53.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PUMA/BBC3 using anti-PUMA/BBC3 antibody (A04899-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PUMA/BBC3 antigen affinity purified polyclonal antibody (Catalog # A04899-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PUMA/BBC3 at approximately 18 kDa. The expected band size for PUMA/BBC3 is at 18 kDa.
Gene/Protein Information For BBC3 (Source: Uniprot.Org, NCBI)
Bcl-2-binding component 3, isoforms 1/2
BCL2 binding component 3; bcl-2-binding component 3; JFY1; JFY-1; p53 up-regulated modulator of apoptosis; PUMAFLJ42994 BBC3 JFY-1, JFY1, PUMA BCL2 binding component 3 bcl-2-binding component 3|p53 up-regulated modulator of apoptosis|p53-upregulated modulator of apoptosis*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on BBC3, check out the BBC3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for BBC3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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