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Product Info Summary
| SKU: | M05260 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Pygopus 2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M05260
BM3982 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Pygopus 2 Rabbit Monoclonal Antibody catalog # M05260. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Pygopus 2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05260)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABI-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Pygopus 2
Reactive Species
M05260 is reactive to PYGO2 in Human, Mouse
Observed Molecular Weight
50 kDa
Calculated molecular weight
41.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05260 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
Positive Control
WB: human Hela whole cell, human MCF-7 whole cell, human SH-SY5Y whole cell, human PC-3 whole cell, huamn U2OS whole cell, mouse NIH/3T3 whole cell
IHC: human uterus tissue
Validation Images & Assay Conditions
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Western blot analysis of Pygopus 2 using anti-Pygopus 2 antibody (M00283).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human SH-SY5Y whole cell lysates,
Lane 4: human PC-3 whole cell lysates,
Lane 5: huamn U2OS whole cell lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Pygopus 2 antigen affinity purified monoclonal antibody (M00283) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Pygopus 2 at approximately 50 kDa. The expected band size for Pygopus 2 is at 41 kDa.
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Immunohistochemical analysis of paraffin-embedded human uterus, using Pygopus 2 Antibody.
Specific Publications For Anti-Pygopus 2 Rabbit Monoclonal Antibody (M05260)
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5 Customer Q&As for Anti-Pygopus 2 Rabbit Monoclonal Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Pygopus 2 Rabbit Monoclonal antibody M05260. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-23
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-23
Question
Would anti-Pygopus 2 Rabbit Monoclonal antibody M05260 work on dog WB with nasal cavity epithelium?
Verified Customer
Verified customer
Asked: 2018-07-16
Answer
Our lab technicians have not tested anti-Pygopus 2 Rabbit Monoclonal antibody M05260 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Pygopus 2 Rabbit Monoclonal antibody M05260 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog nasal cavity epithelium in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-16
Question
I am interested in to test anti-Pygopus 2 Rabbit Monoclonal antibody M05260 on human liver for research purposes, then I may be interested in using anti-Pygopus 2 Rabbit Monoclonal antibody M05260 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-07-05
Answer
The products we sell, including anti-Pygopus 2 Rabbit Monoclonal antibody M05260, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-07-05
Question
Is there a BSA free version of anti-Pygopus 2 Rabbit Monoclonal antibody M05260 available?
A. Roberts
Verified customer
Asked: 2015-12-28
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Pygopus 2 Rabbit Monoclonal antibody M05260 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-12-28
Question
We are currently using anti-Pygopus 2 Rabbit Monoclonal antibody M05260 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on canine tissues as well?
L. Rodriguez
Verified customer
Asked: 2013-06-03
Answer
The anti-Pygopus 2 Rabbit Monoclonal antibody (M05260) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-06-03
