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Product Info Summary
| SKU: | A30504 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-QORX TP53I3 Antibody
SKU/Catalog Number
A30504
Size
100ul
Form
Liquid
Description
Boster Bio Anti-QORX TP53I3 Antibody (Catalog# A30504). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-QORX TP53I3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30504)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human QORX. Immunogen sequence location: 241-290
Reactive Species
A30504 is reactive to TP53I3 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
35.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30504 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:20000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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Western Blot analysis of various cells using PIG3 Polyclonal Antibody
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Western blot analysis of lysates from 293 cells, using QORX Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-QORX TP53I3 Antibody (A30504)
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Customer Q&As
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4 Customer Q&As for Anti-QORX TP53I3 Antibody
Question
Here is the WB image, lot number and protocol we used for gastric mucosa using anti-QORX antibody A30504. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-27
Question
My question regarding product A30504, anti-QORX antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-09-07
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30504 anti-QORX antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-09-07
Question
Does anti-QORX antibody A30504 work for WB with gastric mucosa?
O. Lewis
Verified customer
Asked: 2016-08-08
Answer
According to the expression profile of gastric mucosa, TP53I3 is highly expressed in gastric mucosa. So, it is likely that anti-QORX antibody A30504 will work for WB with gastric mucosa.
Boster Scientific Support
Answered: 2016-08-08
Question
We are currently using anti-QORX antibody A30504 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?
L. Parker
Verified customer
Asked: 2013-09-23
Answer
The anti-QORX antibody (A30504) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-09-23
