Anti-Quiescin Q6/QSOX1 Antibody Picoband™

Product Name

Anti-Quiescin Q6/QSOX1 Antibody Picoband™

View all QSOX1/Quiescin Q6 Antibodies

SKU/Catalog Number

A04549-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-Quiescin Q6/QSOX1 Antibody Picoband™ catalog # A04549-1. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Quiescin Q6/QSOX1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04549-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Quiescin Q6/QSOX1 recombinant protein (Position: R32-Q421).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A04549-1 is reactive to QSOX1 in Human

Applications

A04549-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

105 kDa

Calculated molecular weight

82.578kDa

Background of QSOX1/Quiescin Q6

Sulfhydryl oxidase 1 is an enzyme that in humans is encoded by the QSOX1 gene. This gene encodes a protein that contains domains of thioredoxin and ERV1, members of two long-standing gene families. The gene expression is induced as fibroblasts begin to exit the proliferative cycle and enter quiescence, suggesting that this gene plays an important role in growth regulation. Two transcript variants encoding two different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Quiescin Q6/QSOX1 using anti-Quiescin Q6/QSOX1 antibody (A04549-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human U251 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Quiescin Q6/QSOX1 antigen affinity purified polyclonal antibody (Catalog # A04549-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Quiescin Q6/QSOX1 at approximately 105 kDa. The expected band size for Quiescin Q6/QSOX1 is at 83 kDa.

Gene/Protein Information For QSOX1 (Source: Uniprot.org, NCBI)

Alternative Names

EC 1.8.3.2; FLJ34858; HQSOX; Q6; QSCN6; QSOX1; quiescin Q6 sulfhydryl oxidase 1; Quiescin Q6; sulfhydryl oxidase 1; Sulfhydryl Oxidase-1 QSOX1 Q6, QSCN6 quiescin sulfhydryl oxidase 1 sulfhydryl oxidase 1|quiescin Q6 sulfhydryl oxidase 1|testis tissue sperm-binding protein Li 62n|thiol oxidase 1

For more info on QSOX1, check out the QSOX1 Infographic

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In this infographic, you will see the following information for QSOX1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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