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Product Info Summary
| SKU: | M04117 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, Flow Cytometry, ICC, WB |
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Product info
Product Name
Anti-R-RAS2 Purified Monoclonal Antibody
SKU/Catalog Number
M04117
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-R-RAS2 Purified Monoclonal Antibody (Catalog# M04117). Tested in Flow Cytometry, WB, ELISA, ICC application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-R-RAS2 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04117)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
EM-50
Isotype
Mouse IgG1 kappa
Immunogen
Human TC21. The mouse monoclonal antibody EM-50 recognizes R-Ras2 / TC21 protein (intracellular antigen) and does not react with R-Ras1, H-Ras, K-Ras, and N-Ras.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M04117 is reactive to RRAS2 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
23.4 kDa
Background of RRAS2
R-Ras2 / TC21 is the only member of R-Ras family of small GTPases that shows transforming activities similar to H-Ras, N-Ras, and K-Ras, and it is also structurally similar to them. R-Ras2 seems to play an important role in activating signal transduction pathways that control cell proliferation. Its mutations are associated with the growth of certain tumors, but also overexpression of the wild type form of R-Ras2 has been frequently detected in various carcinomas. Pseudogenes of R-Ras2 gene are found on chromosomes 1 and 2. Alternate splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04117 is guaranteed for ELISA, Flow Cytometry, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 μg/ml. Intracellular staining.
Validation Images & Assay Conditions
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Flow cytometry analysis (intracellular staining) of R-Ras2/TC21 in HEK-293 R-Ras2-GFP transfectants using monoclonal antibody EM-50, purified / GAM-APC.
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Immunocytochemistry analysis of colocalization of R-Ras2/TC21 signal (red) and GFP signal (green) in COS cells transfected with R-Ras2/TC21-GFP construct, after fixation and permeabilization. R-Ras2/TC21 was detected using monoclonal antibody EM-50, purified.
Specific Publications For Anti-R-RAS2 Purified Monoclonal Antibody (M04117)
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