Click image to see more details
Product Info Summary
| SKU: | M04117 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, Flow Cytometry, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-R-RAS2 Purified Monoclonal Antibody
View all TC21/R-Ras2 Antibodies
SKU/Catalog Number
M04117
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-R-RAS2 Purified Monoclonal Antibody (Catalog# M04117). Tested in Flow Cytometry, WB, ELISA, ICC application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-R-RAS2 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04117)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
EM-50
Isotype
Mouse IgG1 kappa
Immunogen
Human TC21. The mouse monoclonal antibody EM-50 recognizes R-Ras2 / TC21 protein (intracellular antigen) and does not react with R-Ras1, H-Ras, K-Ras, and N-Ras.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M04117 is reactive to RRAS2 in Human
Applications
M04117 is guaranteed for ELISA, Flow Cytometry, ICC, WB Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
23.4kDa
Background of TC21/R-Ras2
R-Ras2 / TC21 is the only member of R-Ras family of small GTPases that shows transforming activities similar to H-Ras, N-Ras, and K-Ras, and it is also structurally similar to them. R-Ras2 seems to play an important role in activating signal transduction pathways that control cell proliferation. Its mutations are associated with the growth of certain tumors, but also overexpression of the wild type form of R-Ras2 has been frequently detected in various carcinomas. Pseudogenes of R-Ras2 gene are found on chromosomes 1 and 2. Alternate splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 μg/ml. Intracellular staining.
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Flow Cytometry validation of RRAS2 using Anti-R-RAS2 Purified Monoclonal Antibody (M04117).
Flow cytometry analysis (intracellular staining) of R-Ras2/TC21 in HEK-293 R-Ras2-GFP transfectants using monoclonal antibody EM-50
Click image to see more details
Figure 1. Immunocytochemistry staining of RRAS2 using Anti-R-RAS2 Purified Monoclonal Antibody (M04117).
Immunocytochemistry analysis of colocalization of R-Ras2/TC21 signal (red) and GFP signal (green) in COS cells transfected with R-Ras2/TC21-GFP constructProtein Target Info & Infographic
Gene/Protein Information For RRAS2 (Source: Uniprot.org, NCBI)
Gene Name
RRAS2
Full Name
Ras-related protein R-Ras2
Weight
23.4kDa
Superfamily
small GTPase superfamily
Alternative Names
ras-related protein R-Ras2; related RAS viral (r-ras) oncogene homolog 2; RRAS2; TC21; TC21Ras-like protein TC21; Teratocarcinoma oncogene RRAS2 NS12, TC21 RAS related 2 ras-related protein R-Ras2|ras-like protein TC21|related RAS viral (r-ras) oncogene homolog 2|teratocarcinoma oncogene
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RRAS2, check out the RRAS2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RRAS2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-R-RAS2 Purified Monoclonal Antibody (M04117)
Hello CJ!
No publications found for M04117
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-R-RAS2 Purified Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-R-RAS2 Purified Monoclonal Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
