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Product Info Summary
| SKU: | M03588 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-Rab 10 Monoclonal Antibody
SKU/Catalog Number
M03588
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Rab 10 Monoclonal Antibody catalog # M03588. Tested in ELISA, IF, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rab 10 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03588)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
4E2
Isotype
IgG
Immunogen
Purified recombinant fragment of human Rab 10 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M03588 is reactive to RAB10 in Human, Mouse
Applications
M03588 is guaranteed for ELISA, IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
22.541kDa
Background of RAB10
Anti Mesothelin Antibody recognizes Mesothelin that is a glycosyl-phosphatidylinositol–anchored glycoprotein present on the cell surface of various human solid tumors. The mesothelin (MSLN) gene encodes a 71-kDa precursor protein that is processed to a 40-kDa glycosylphosphatidylinositol–anchored protein that composes the mature portion and an NH2 terminal 31-kDa fragment called megakaryocyte-potentiating factor that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues, such as mesothelium, but aberrantly over expressed in mesotheliomas, ovarian, and pancreatic cancers. The biological functions of mesothelin remain elusive. A recent study showed that mesothelin binds to MUC16/CA125, and that this interaction mediates cell adhesion, suggesting that there may be an important role for MUC16/CA125 and mesothelin in the metastatic spread of ovarian cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
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Figure 2. Immunofluorescent staining data of RAB10 using Anti-Rab 10 Monoclonal Antibody (M03588).
Immunofluorescence (IF) analysis of LOVO cells using Rab 10 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye.
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Figure 1. Western blotting validation for Anti-Rab 10 Monoclonal Antibody M03588
Western Blot (WB) analysis using Rab 10 Monoclonal Antibody against HeLa (1) and NIH/3T3 (2) cell lysate.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For RAB10 (Source: Uniprot.org, NCBI)
Gene Name
RAB10
Full Name
Ras-related protein Rab-10
Weight
22.541kDa
Superfamily
small GTPase superfamily
Alternative Names
GTP-binding protein RAB10; RAB10, member RAS oncogene family; ras-related GTP-binding protein; ras-related protein Rab-10 RAB10 RAB10, member RAS oncogene family ras-related protein Rab-10|GTP-binding protein RAB10|ras-related GTP-binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAB10, check out the RAB10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAB10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rab 10 Monoclonal Antibody (M03588)
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No publications found for M03588
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Customer Reviews
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