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Product Info Summary
| SKU: | A04231-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-RAB14 Antibody Picoband®
SKU/Catalog Number
A04231-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-RAB14 Antibody Picoband® catalog # A04231-1. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-RAB14 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04231-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived human RAB14 recombinant protein (Position: D108-C215).
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A04231-1 is reactive to RAB14 in Human, Mouse, Rat
Observed Molecular Weight
24 kDa
Calculated molecular weight
23.9 kDa
Background of RAB14
Ras-related protein Rab-14 is a protein that in humans is encoded by the RAB14 gene. It is mapped to 9q33.2 based on an alignment of the RAB14 sequence with the genomic sequence. RAB14 belongs to the large RAB family of low molecular mass GTPases that are involved in intracellular membrane trafficking. These proteins act as molecular switches that flip between an inactive GDP-bound state and an active GTP-bound state in which they recruit downstream effector proteins onto membranes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04231-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, human PC-3 whole cell, human T-47D whole cell, human A549 whole cell, human A431 whole cell, human U937 whole cell, human MCF-7 whole cell, rat brain tissue, mouse brain tissue, mouse lung tissue
FCM: Hela cell
Validation Images & Assay Conditions
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Western blot analysis of RAB14 using anti-RAB14 antibody (A04231-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human T-47D whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: human A431 whole cell lysates,
Lane 6: human U937 whole cell lysates,
Lane 7: human MCF-7 whole cell lysates,
Lane 8: rat brain tissue lysates,
Lane 9: mouse brain tissue lysates,
Lane 10: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB14 antigen affinity purified polyclonal antibody (Catalog # A04231-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB14 at approximately 24 kDa. The expected band size for RAB14 is at 24 kDa.
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Flow Cytometry analysis of Hela cells using anti-RAB14 antibody (A04231-1).
Overlay histogram showing Hela cells stained with A04231-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB14 Antibody (A04231-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Specific Publications For Anti-RAB14 Antibody Picoband® (A04231-1)
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