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Product Info Summary
| SKU: | M04589-1 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-RAB1B Antibody
SKU/Catalog Number
M04589-1
Size
50 µl
Description
Boster Bio Anti-RAB1B Antibody (Catalog # M04589-1). Tested in WB application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-RAB1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04589-1)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1673CT571.17.71
Isotype
IgG1,k
Immunogen
This RAB1B antibody is generated from a mouse immunized with a recombinant protein between 1-201 amino acids from human RAB1B.
Reactive Species
M04589-1 is reactive to RAB1B in Human, Mouse, Rat
Calculated molecular weight
22.2 kDa
Background of RAB1B
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB1B regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments. Plays a role in the initial events of the autophagic vacuole development which take place at specialized regions of the endoplasmic reticulum.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04589-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:4000
Validation Images & Assay Conditions
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All lanes : Anti-RAB1B Antibody at 1:4000 dilution
Lane 1: Hela whole cell lysate
Lane 2: A431 whole cell lysate
Lane 3: U-20S whole cell lysate
Lane 4: rat liver lysate
Lane 5: NIH/3T3 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 22 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-RAB1B Antibody (M04589-1)
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